Substituted titanocenes induce caspase-dependent apoptosis in human epidermoid carcinoma cells in vitro and exhibit antitumour activity in vivo
| Autor: | Amanda O'Neill, Iduna Fichtner, Nigel J. Sweeney, John H. Bannon, Matthias Tacke, Katja Strohfeldt, R.W.G. Watson, Margaret M. Mc Gee, Clara Pampillón |
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| Jazyk: | angličtina |
| Rok vydání: | 2007 |
| Předmět: |
Cancer Research
Programmed cell death Blotting Western cisplatin Mice Nude Antineoplastic Agents Biology chemotherapy Inhibitory Concentration 50 Mice medicine Organometallic Compounds Tumor Cells Cultured Cytotoxic T cell Animals Humans neoplasms titanocene Cell Proliferation Cisplatin Titanium Cell growth Caspase 3 Body Weight Cell Cycle apoptosis Oncology Epidermoid carcinoma epidermoid cancer caspases Apoptosis Cancer cell Immunology Cancer research Carcinoma Squamous Cell Female Translational Therapeutics A431 cells medicine.drug HeLa Cells |
| Zdroj: | British Journal of Cancer |
| ISSN: | 1532-1827 0007-0920 |
| Popis: | Titanocene compounds are a novel series of agents that exhibit cytotoxic effects in a variety of human cancer cells in vitro and in vivo. In this study, the antiproliferative activity of two titanocenes (Titanocenes X and Y) was evaluated in human epidermoid cancer cells in vitro. Titanocenes X and Y induce apoptotic cell death in epidermoid cancer cells, with IC50 values that are comparable to cisplatin. Characterisation of the cell death pathway induced by titanocene compounds in A431 cells revealed that apoptosis is preceded by cell cycle arrest and the inhibition of cell proliferation. The induction of apoptosis is dependent on the activation of caspase-3 and -7 but not caspase-8. Furthermore, the antitumour activity of Titanocene Y was tested in an A431 xenograft model of epidermoid cancer. Results indicate that Titanocene Y significantly reduced the growth of A431 xenografts with an antitumour effect similar to cisplatin. These results suggest that titanocenes represent a novel series of promising antitumour agents. |
| Databáze: | OpenAIRE |
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