Experimental Model of Human Malignant Mesothelioma in Athymic Mice

Autor: David Cottet-Dumoulin, Anna Faivre, Didier J. Colin, Stéphane Germain, Véronique Serre-Beinier, Frédéric Triponez
Jazyk: angličtina
Rok vydání: 2018
Předmět:
0301 basic medicine
Mesothelioma
Lung Neoplasms
Carcinogenesis
Cell
orthotopic xenotransplantation
Athymic mouse
Cell Count
lcsh:Chemistry
0302 clinical medicine
Medicine
Macrophage
lcsh:QH301-705.5
Spectroscopy
Cancer
education.field_of_study
ddc:617
Immune cells
General Medicine
respiratory system
Body Fluids
Computer Science Applications
Gene Expression Regulation
Neoplastic
medicine.anatomical_structure
pleura
030220 oncology & carcinogenesis
mesothelioma
Pleura
medicine.symptom
Epithelial-Mesenchymal Transition
Cell Survival
Population
athymic mouse
Mice
Nude
Inflammation
Orthotopic xenotransplantation
Article
Catalysis
Inorganic Chemistry
03 medical and health sciences
Immune system
immune cells
Cell Line
Tumor
Animals
Humans
cancer
Physical and Theoretical Chemistry
education
Molecular Biology
business.industry
Macrophages
Mesothelioma
Malignant
Organic Chemistry
medicine.disease
Xenograft Model Antitumor Assays
respiratory tract diseases
030104 developmental biology
lcsh:Biology (General)
lcsh:QD1-999
Cancer research
business
Zdroj: International Journal of Molecular Sciences
Volume 19
Issue 7
International Journal of Molecular Sciences, Vol. 19, No 7 (2018) P. 1881
International Journal of Molecular Sciences, Vol 19, Iss 7, p 1881 (2018)
ISSN: 1422-0067
1661-6596
DOI: 10.3390/ijms19071881
Popis: Malignant pleural mesothelioma (MPM) is a thoracic aggressive cancer caused by asbestos exposure, which is difficult to diagnose and treat. Here, we characterized an in vivo orthotopic xenograft model consisting of human mesothelioma cells (designed as H2052/484) derived from a pleural NCI-H2052 tumor injected in partially immunodeficient athymic mice. We assessed tumor formation and tumor-dependent patterns of inflammation. H2052/484 cells conserved their mesothelioma phenotype and most characteristics from the parental NCI-H2052 cells. After intra-thoracic injection of H2052/484 cells, thoracic tumors developed in nearly all mice (86%) within 14 days, faster than from parental NCI-H2052 cells. When the mice were euthanized, the pleural lavage fluid was examined for immune cell profiles. The pleural immune cell population increased with tumor development. Interestingly, the proportion of myeloid-derived suppressor cell and macrophage (especially CD206+ M2 macrophages) populations increased in the pleural fluid of mice with large mesothelioma development, as previously observed in immunocompetent mice. This reliable orthotopic model recapitulates human mesothelioma and may be used for the study of new treatment strategies.
Databáze: OpenAIRE
Externí odkaz: