New human myelodysplastic cell line, TER-3: G-CSF specific downregulation of Ca2+/calmodulin-dependent protein kinase IV
| Autor: | Nobuyuki Mizunuma, Masaki Mori, Takashi Kato, Hirotoshi Hayasawa, Muneo Yamada, Kiyohiko Hatake, Yukihito Ishizaka, Kazuma Ikeda, Akira Miyazato, Misa Katsuyama, Yuji Mishima, Yuko Mishima, Hiroshi Tomizuka, Toshiyuki Takizawa, Keiya Ozawa, Yasuhito Terui, Masuzu Ueda |
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| Rok vydání: | 2002 |
| Předmět: |
Male
Myeloid Physiology Clinical Biochemistry CD33 Cell Culture Techniques CD34 Down-Regulation Bone Marrow Cells Biology CD19 Cell Line Downregulation and upregulation Granulocyte Colony-Stimulating Factor medicine Humans RNA Messenger Protein kinase A Cell Size Dose-Response Relationship Drug Cell Differentiation DNA Cell Biology Middle Aged Molecular biology Cell biology medicine.anatomical_structure Multipotent Stem Cell Cell culture Karyotyping Myelodysplastic Syndromes Antigens Surface Calcium-Calmodulin-Dependent Protein Kinases biology.protein Interleukin-3 Calcium-Calmodulin-Dependent Protein Kinase Type 4 Cell Division |
| Zdroj: | Journal of Cellular Physiology. 191:183-190 |
| ISSN: | 1097-4652 0021-9541 |
| Popis: | We have established a new hematopoietic cell line from a patient with myelodysplastic syndrome (MDS), which was refractory anemia with excess blasts (RAEB). This cell line, designated TER-3, depends on several cytokines for long-term survival and growth, and requires interleukin-3 (IL-3) for continuous growth. Cytochemical analysis revealed that TER-3 cells are weakly dianisidine positive and nonspecific esterase positive, but peroxidase negative. The surface marker profile shows that the TER-3 cells are strongly positive for myeloid, lymphoid, and megakaryocytic antigens such as CD15, CD19, and CD61, and negative for some common multilineage antigens such as CD13, CD33, and CD34. Thus, this cell line has a multilineage phenotype, suggesting that the transformation event occurred in multipotent stem cells. Dianisidine- and nonspecific esterase-positive TER-3 cells increase with granulocyte-colony stimulating factor (G-CSF) rather than with IL-3. These results suggest that the cell line is useful for understanding the mechanism underlying G-CSF-associated hematopoietic cell differentiation and activation in the patient with MDS. |
| Databáze: | OpenAIRE |
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