Diagnosis of ovarian tumour tissues by SR-FTIR spectroscopy : a pilot study
| Autor: | M. Grzelak, Pawel Wrobel, Dariusz Adamek, Marek Lankosz, Zdzislaw Stegowski, Hiram Castillo-Michel, Ł. Chmura, Bernhard Hesse |
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| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Pathology medicine.medical_specialty Molecular composition Infrared spectroscopy Pilot Projects Vibration Analytical Chemistry 03 medical and health sciences Biological specimen Spectroscopy Fourier Transform Infrared medicine Humans Ovarian tumours Fourier transform infrared spectroscopy Instrumentation Spectroscopy Ovarian Neoplasms Chemistry Cancer Histology medicine.disease Atomic and Molecular Physics and Optics 030104 developmental biology Female Ovarian cancer |
| Popis: | Ovarian cancer is the seventh most common cancer among women across the world with very high mortality rates. Histology is considered the gold standard for tumour diagnosis. FTIR spectroscopy is relies on registering biochemical differences in the samples analysed, including biological specimens. Therefore, the Synchrotron radiation based-Fourier transform infrared spectroscopy (SR-FTIR) was used for the preliminary investigation of the molecular composition of the human, non-fixed ovarian neoplastic tissues with different type of biological potential. The study that was carried out on thin tissue sections, placed on barium fluoride infrared windows, was focused on investigating spatial distribution of the biochemical markers in various ovarian tumours. Since the structural constituents of tissues accumulate different molecules which may correspond to the specific type of ovarian tumours, the main goal of this study was to check if the mean intensities of the spectral lines of some bio-molecules can be treated as ovarian cancer bio-indicators. Moreover, an attempt to identify and understand the underlying biochemical changes associated with the disease was carried out. The major spectral differences in the frequency and intensities were identified as bonds of lipids, protein massif and nucleic acids. The results obtained suggest that Fourier transform infrared spectroscopy can be used as a supporting tool in the analysis of neoplastic ovarian tissue. |
| Databáze: | OpenAIRE |
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