Replacement of Ribosomal Protein S1 by Interference Factor iα in Ribosomal Binding of Phage MS2 RNA
| Autor: | Wlodzimierz Szer, José Miguel Hermoso |
|---|---|
| Rok vydání: | 1974 |
| Předmět: |
Poly U
Multidisciplinary Polynucleotides RNA Ribosomal RNA Biology Tritium Ribosome Viral Proteins Bacterial Proteins Biochemistry Ribosomal protein Protein Biosynthesis 28S ribosomal RNA Escherichia coli Protein biosynthesis RNA Viral Initiation factor Electrophoresis Polyacrylamide Gel 30S Biological Sciences: Biochemistry Carbon Radioisotopes RNA Messenger Peptide Chain Initiation Translational Ribosomes |
| Zdroj: | Proceedings of the National Academy of Sciences. 71:4708-4712 |
| ISSN: | 1091-6490 0027-8424 |
| DOI: | 10.1073/pnas.71.12.4708 |
| Popis: | The MS2 RNA binding capacity of 30S ribosomal subunits, which is lost when protein S1 is removed, can be restored following incubation with interference factor iα and repelleting. Polyacrylamide-agarose gel electrophoresis shows that, under these conditions, a faster moving, non-RNA binding 30S species, which contains no S1, is converted to a slower moving RNA-binding one, having the same mobility as the 30S species that contains protein S1. Factor iα binds to single-stranded RNAs in a pattern that closely resembles the RNA binding pattern of initiation factor IF-3. |
| Databáze: | OpenAIRE |
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