N-Glycolyl GM1 Ganglioside as a Receptor for Simian Virus 40▿ †
| Autor: | Ten Feizi, Helge Ewers, María Asunción Campanero-Rhodes, Alicia E. Smith, Robert A. Childs, Yibing Zhang, Anne Imberty, Sandro Sonnino, Laura Mauri, Wengang Chai, Ari Helenius |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2007 |
| Předmět: |
Models
Molecular Glycan Receptor expression viruses Immunology Virus Attachment G(M1) Ganglioside Simian virus 40 Microbiology Virus Cell Line Mice Glycolipid Cell surface receptor Virology Animals Receptor Ganglioside biology Ligand (biochemistry) Molecular biology Virus-Cell Interactions Protein Structure Tertiary carbohydrates (lipids) Insect Science biology.protein Receptors Virus lipids (amino acids peptides and proteins) Capsid Proteins Protein Binding |
| Popis: | Carbohydrate microarrays have emerged as powerful tools in analyses of microbe-host interactions. Using a microarray with 190 sequence-defined oligosaccharides in the form of natural glycolipids and neoglycolipids representative of diverse mammalian glycans, we examined interactions of simian virus 40 (SV40) with potential carbohydrate receptors. While the results confirmed the high specificity of SV40 for the ganglioside GM1, they also revealed that N -glycolyl GM1 ganglioside [GM1(Gc)], which is characteristic of simian species and many other nonhuman mammals, is a better ligand than the N -acetyl analog [GM1(Ac)] found in mammals, including humans. After supplementing glycolipid-deficient GM95 cells with GM1(Ac) and GM1(Gc) gangliosides and the corresponding neoglycolipids with phosphatidylethanolamine lipid groups, it was found that GM1(Gc) analogs conferred better virus binding and infectivity. Moreover, we visualized the interaction of NeuGc with VP1 protein of SV40 by molecular modeling and identified a conformation for GM1(Gc) ganglioside in complex with the virus VP1 pentamer that is compatible with its presentation as a membrane receptor. Our results open the way not only to detailed studies of SV40 infection in relation to receptor expression in host cells but also to the monitoring of changes that may occur with time in receptor usage by the virus. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|