Recombinant Lactobacillus casei Expressing Capsid Protein VP60 can Serve as Vaccine Against Rabbit Hemorrhagic Disease Virus in Rabbits
Autor: | Tiantian Guo, Yi Ru, Yigang Xu, Yijing Li, Lijie Tang, Xinyuan Qiao, Li Wang, Wen Cui, Tian Xia, Yanping Jiang, Han Zhou |
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Rok vydání: | 2019 |
Předmět: |
0301 basic medicine
Lactobacillus casei 030106 microbiology Immunology lcsh:Medicine lactobacillus casei (l. casei) Immunofluorescence Article Virus law.invention 03 medical and health sciences Probiotic Immune system law vaccine Drug Discovery medicine Pharmacology (medical) Pharmacology biology medicine.diagnostic_test lcsh:R biology.organism_classification Fusion protein Virology 030104 developmental biology Infectious Diseases Capsid Lactobacillus casei (L. casei) rabbit hemorrhagic disease virus (rhdv) Recombinant DNA vp60(vp1) |
Zdroj: | Vaccines, Vol 7, Iss 4, p 172 (2019) Vaccines Volume 7 Issue 4 |
ISSN: | 2076-393X |
Popis: | Rabbit hemorrhagic disease virus (RHDV) is the causative agent of rabbit hemorrhagic disease (RHD). RHD, characterized by hemorrhaging, liver necrosis, and high morbidity and mortality in rabbits and hares, causes severe economic losses in the rabbit industry worldwide. Due to the lack of an efficient in-vitro propagation system for RHDV, the current vaccine is produced via chemical inactivation of crude RHDV preparation derived from the livers of infected rabbits. Inactivated vaccines are effective for controlling RHD, but the potential problems of biosafety and animal welfare have negative effects on the application of inactivated vaccines. In this study, an oral Lactobacillus casei (L. casei) vaccine was used as an antigen delivery system to express RHDV capsid protein VP60(VP1)-eGFP fusion protein. The expression of the recombinant protein was confirmed via western blotting and immunofluorescence (IFA). Our results indicate that oral administration of this probiotic vaccine can stimulate secretory immunoglobulin A (SIgA)-based mucosal and IgG-based humoral immune responses in rabbits. The immunized rabbits were completely protected against challenge with RHDV. Our findings indicate that the L. casei expression system is a new strategy for the development of a safe and efficient vaccine against RHDV. |
Databáze: | OpenAIRE |
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