Cytotoxicity of Mycoplasma mycoides subsp. mycoides small colony type to bovine epithelial cells
| Autor: | Carole Janis, Giuseppe Bertoni, Joachim Frey, Daniela F. Bischof, Edy M. Vilei |
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| Rok vydání: | 2007 |
| Předmět: |
Glycerol
Immunology Molecular Sequence Data Virulence Glycerolphosphate Dehydrogenase Biology medicine.disease_cause Microbiology Bacterial Adhesion Contagious bovine pleuropneumonia Bacterial Proteins medicine Cytotoxic T cell Animals Cytotoxicity Cellular Microbiology: Pathogen-Host Cell Molecular Interactions Mycoplasma mycoides Epithelial Cells Mycoplasma Gene Expression Regulation Bacterial Hydrogen Peroxide medicine.disease biology.organism_classification In vitro Oxidative Stress Infectious Diseases Genes Bacterial Mollicutes Parasitology Cattle |
| Zdroj: | Infection and immunity. 76(1) |
| ISSN: | 1098-5522 |
| Popis: | The cytotoxicities of various strains of Mycoplasma mycoides subsp. mycoides small colony type (SC), the agent of contagious bovine pleuropneumonia (CBPP), were measured in vitro using embryonic calf nasal epithelial (ECaNEp) cells. Strains isolated from acute cases of CBPP induced high cytotoxicity in the presence of glycerol, concomitant with the release of large amounts of toxic H 2 O 2 that were found to be translocated into the cytoplasms of the host cells by close contact of the Mycoplasma strains with the host cells. Currently used vaccine strains also showed high cytotoxicity and high H 2 O 2 release, indicating that they are attenuated in another virulence attribute. Strains isolated from recent European outbreaks of CBPP with mild clinical signs, which are characterized by a defect in the glycerol uptake system, released small amounts of H 2 O 2 and showed low cytotoxicity to ECaNEp cells. M. mycoides subsp. mycoides SC strain PG1 released large amounts of H 2 O 2 but was only slightly cytotoxic. PG1 was found to have a reduced capacity to bind to ECaNEp cells and was unable to translocate H 2 O 2 into the bovine cells, in contrast to virulent strains that release large amounts of H 2 O 2 . Thus, an efficient translocation of H 2 O 2 into host cells is a prerequisite for the cytotoxic effect and requires an intact adhesion mechanism to ensure a close contact between mycoplasmas and host cells. |
| Databáze: | OpenAIRE |
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