Alendronate alters osteoblast activities
| Autor: | Tormod B Krüger, Maria A. Landin, Janne E. Reseland, Bente Brokstad Herlofson |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
Leptin
medicine.medical_specialty Cell Survival Osteocalcin Cell Culture Techniques Bone and Bones Collagen Type I 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Internal medicine Lactate dehydrogenase medicine Cytotoxic T cell Humans Secretion Viability assay General Dentistry Chemokine CCL5 Cells Cultured Cell Proliferation Messenger RNA Osteoblasts biology Alendronate Bone Density Conservation Agents L-Lactate Dehydrogenase Chemistry Interleukin-8 Osteoblast Cell Differentiation 030206 dentistry General Medicine Alkaline Phosphatase Molecular biology Collagen Type I alpha 1 Chain Endocrinology medicine.anatomical_structure 030220 oncology & carcinogenesis biology.protein Alkaline phosphatase |
| Zdroj: | Acta odontologica Scandinavica. 74(7) |
| ISSN: | 1502-3850 |
| Popis: | Due to accumulation in the bone matrix and a half-life of at least 10 years, it is important to understand the cellular impact of bisphosphonates (BPs). This study assessed the effects of alendronate (ALN) on human primary osteoblasts.Osteoblasts were incubated with ALN (5, 20 and 100 μM), and both cells and cell culture media were harvested after d 1, 3, 7 or 14. Proliferation was evaluated byALN had no significant effects on cell viability. The lower concentrations enhanced the proliferation, whereas 100 μM diminished the proliferation. mRNA expression of osteocalcin (OC), alkaline phosphatase (ALP) and α-1 type 1 collagen were reduced, whereas ALN enhanced the expression of leptin mRNA and the secretion of interleukin-8 (IL-8) and regulated on activation normal T cell expressed and secreted (RANTES).ALN enhanced the secretion of immune factors from human osteoblasts. Combined with a lower rate of proliferation and a decline in differentiation, this indicates that higher dosages or accumulation may cause undesirable local changes in bone. |
| Databáze: | OpenAIRE |
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