Designing a biochip following multiplex polymerase chain reaction for the detection of Salmonella serovars Typhimurium, Enteritidis, Infantis, Hadar, and Virchow in poultry products
Autor: | Hsin-Yen Chen, Chia-Ming Shih, Hau-Yang Tsen, Yu-Cheng Chiang, Chien Ku Lin, Hsien-Huang Wang, Latha Ramireddy |
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Rok vydání: | 2017 |
Předmět: |
0301 basic medicine
Serotype Salmonella typhimurium Salmonella Food Safety 030106 microbiology lcsh:TX341-641 medicine.disease_cause Serogroup law.invention Microbiology 03 medical and health sciences law Lab-On-A-Chip Devices Multiplex polymerase chain reaction medicine media_common.cataloged_instance Animals European union Poultry Products Polymerase chain reaction media_common Pharmacology biology Hybridization probe lcsh:RM1-950 primers for Salmonella Virchow Salmonella enterica Equipment Design biology.organism_classification Virology lcsh:Therapeutics. Pharmacology 030104 developmental biology Food Microbiology Flock biochip for five Salmonella serovars lcsh:Nutrition. Foods and food supply Multiplex Polymerase Chain Reaction Food Science |
Zdroj: | Journal of Food and Drug Analysis, Vol 26, Iss 1, Pp 58-66 (2018) |
ISSN: | 1021-9498 |
DOI: | 10.1016/j.jfda.2016.11.019 |
Popis: | Salmonella-contaminated foods, especially poultry-derived foods (eggs, chicken meat), are the major source of salmonellosis. Not only in the European Union (EU), but also in the United States, Japan, and other countries, has salmonellosis been an issue of concern for food safety control agencies. In 2005, EU regulation 1003/2005 set a target for the control and reduction of five target Salmonella enterica serovars— S. Typhimurium, S. Enteritidis, S. Infantis, S. Hadar, and S. Virchow—in breeding flocks. Thus, a simple biochip for the rapid detection of any of these five Salmonella serovars in poultry products may be required. The objectives of this study were to design S. Virchow-specific primers and to develop a biochip for the simultaneous identification of all or any of these five Salmonella serovars in poultry and poultry products. Experimentally, we designed novel polymerase chain reaction (PCR) primers for the specific detection of S. Virchow, S. Infantis, and S. Hadar. The specificity of all these primers and two known primer sets for S. Typhimurium and S. Enteritidis was then confirmed under the same PCR conditions using 57 target strains and 112 nontarget Salmonella strains as well as 103 non- Salmonella strains. Following multiplex PCR, strains of any of these five Salmonella serovars could be detected by a chromogenic biochip deployed with DNA probes specific to these five Salmonella serovars. In comparison with the multiplex PCR methods, the biochip assay could improve the detection limit of each of the Salmonella serovars from N ×10 3 cfu/mL to N ×10 2 cfu/mL sample in either the pure culture or the chicken meat samples. With an 8-hour enrichment step, the detection limit could reach up to N ×10 0 cfu/mL. |
Databáze: | OpenAIRE |
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