Genetic Diversity of HIV-1 GenevifAmong Treatment-Naive Brazilians
| Autor: | Luiz Mario Janini, Fabiola Villanova, Ricardo Sobhie Diaz, Élcio Leal, Marta Barreiros |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
viruses 030106 microbiology Immunology Population Somatic hypermutation HIV Infections Biology medicine.disease_cause law.invention 03 medical and health sciences law Virology vif Gene Products Human Immunodeficiency Virus medicine Humans education Gene Phylogeny Recombination Genetic Genetics Mutation education.field_of_study Genetic diversity Phylogenetic tree Genetic Variation CD4 Lymphocyte Count 030104 developmental biology Infectious Diseases HIV-1 Recombinant DNA Female Viral load Brazil |
| Zdroj: | AIDS Research and Human Retroviruses. 33:952-959 |
| ISSN: | 1931-8405 0889-2229 |
| Popis: | HIV-1 has the Vif protein, which binds to human antiviral proteins APOBEC3 to form complexes to be degraded by cellular proteolysis. To further explore HIV-1 diversity at the population level, we analyzed blood samples from 317 treatment-naive patients in Brazil. In this study, we explored the correlations of Vif polymorphisms with clinical parameters of the patients and found that mutation K22H is associated with low CD4+ cell counts and higher viral loads. Phylogenetic analysis of the vif gene indicated that subtype B was predominant in ∼77% (243/317) of the patients, followed by HIV-1 F ∼18% (56/317), and subtype C ∼4% (12/317); five samples were BF recombinants (∼1% of patients), and one was an AG recombinant. On the basis of the vif gene, we detected the presence of one AG and several previously unknown BF intersubtypes in this population. The global mean diversity, measured by pairwise distances, was 0.0931 ± 0.0006 among sequences of subtype B (n = 243), whereas the mean diversity of subty... |
| Databáze: | OpenAIRE |
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