Lymphoblast-derived hiPS cell lines generated from four individuals of a family of genetically unrelated parents and their female monozygotic twins
| Autor: | Karine Raymond, Christian Freund, Marga J. Bouma, Dorret I. Boomsma, Christine L. Mummery, Adriaan P. IJzerman |
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| Přispěvatelé: | Institut de Recherche Interdisciplinaire de Grenoble (IRIG), Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA), Biological Psychology, APH - Mental Health, APH - Methodology |
| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
Adult
Male Parents 0301 basic medicine Netherlands Twin Register (NTR) [SDV]Life Sciences [q-bio] Induced Pluripotent Stem Cells Germ layer Biology LIN28 Cell Line Kruppel-Like Factor 4 03 medical and health sciences 0302 clinical medicine SOX2 SDG 3 - Good Health and Well-being Humans Lymphocytes lcsh:QH301-705.5 ComputingMilieux_MISCELLANEOUS Aged Genetics Lymphoblast Reproducibility of Results Karyotype Twins Monozygotic Cell Biology General Medicine 030104 developmental biology lcsh:Biology (General) Cell culture KLF4 Female Reprogramming 030217 neurology & neurosurgery Developmental Biology |
| Zdroj: | Bouma, M J, Freund, C, IJzerman, A P, Boomsma, D I, Mummery, C L & Raymond, K 2019, ' Lymphoblast-derived hiPS cell lines generated from four individuals of a family of genetically unrelated parents and their female monozygotic twins ', Stem Cell Research, vol. 41, 101654, pp. 1-5 . https://doi.org/10.1016/j.scr.2019.101654 Stem Cell Research, 41, 101654 Stem Cell Research Stem Cell Research, Elsevier, 2019, 41, pp.101654. ⟨10.1016/j.scr.2019.101654⟩ Stem Cell Research, 41. ELSEVIER Stem Cell Research, Vol 41, Iss, Pp-(2019) Stem Cell Research, 41:101654, 1-5. Elsevier Stem Cell Research, 2019, 41, pp.101654. ⟨10.1016/j.scr.2019.101654⟩ |
| ISSN: | 1876-7753 1873-5061 |
| DOI: | 10.1016/j.scr.2019.101654 |
| Popis: | Lymphoblast cells from four individuals of a family of two genetically unrelated parents and their monozygotic twins were used to generate integration-free human induced pluripotent stem cells (hiPSCs). Reprogramming factors were delivered by co-electroporation of three episomal-based plasmids expressing OCT3/4, SOX2, KLF4, L-MYC and LIN28. The hiPSCs showed a normal karyotype, expressed pluripotency-associated markers, displayed the capacity for in vitro differentiation into the three germ layers and were Epstein Barr virus-free. These hiPSC lines offer the possibility to compare genetically unrelated and genetically identical tissues from different individuals and to study genotype-specific effects, which are particularly relevant for toxicology testing. |
| Databáze: | OpenAIRE |
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