In vivo assessment and characterization of lactic acid bacteria with probiotic profile isolated from human milk powder
| Autor: | Edgar Balcazar López, Rosa Elena Navarro Hernández, José Alfonso Gutiérrez Padilla, Monique Lacroix, Blanca Rosa Aguilar Uscanga, Elisa García Morales, Josué R Solís Pacheco, Ariana Rodríguez Arreola, Flavio Sandoval Garcia |
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| Rok vydání: | 2020 |
| Předmět: |
Blood Glucose
Limosilactobacillus reuteri Male Limosilactobacillus fermentum Lactobacillus fermentum Medicine (miscellaneous) Breast milk Probiotic Bacterial Physiological Phenomena Probiotic activity law.invention chemistry.chemical_compound Feces Mice fluids and secretions Lactobacillus rhamnosus law Lactobacillales Lactic acid bacteria Probiotic bacteria Animals Humans Food science Mexico Mice Inbred BALB C Nutrition and Dietetics biology Milk Human Lacticaseibacillus rhamnosus Probiotics Human milk Spray drying Body Weight food and beverages Spray Drying biology.organism_classification Lactic acid Intestines chemistry Powders Lactobacillus plantarum Bacteria |
| Zdroj: | Nutrición Hospitalaria v.38 n.1 2021 SciELO España: Revistas Científicas Españolas de Ciencias de la Salud Instituto de Salud Carlos III (ISCIII) SciELO España. Revistas Científicas Españolas de Ciencias de la Salud instname |
| ISSN: | 1699-5198 |
| Popis: | espanolIntroduccion: la leche materna (HM) contiene los nutrientes y compuestos bioactivos necesarios para el desarrollo infantil, incluidas bacterias probioticas, que contribuyen a la maduracion intestinal.Objetivo: el objetivo de este estudio fue analizar la viabilidad de las bacterias acidolacticas aisladas de la leche humana con potencial probiotico, despues del proceso de secado, asi como evaluar su posible adhesion en el colon de ratones (BAlb/C) alimentados con leche humana en polvo y leche de una formula comercial.Metodo: se aislaron e identificaron mediante la tecnica de Maldi-Tof-MS las bacterias acidolacticas con posible potencial probiotico en la leche humana en polvo. Se alimento con leche humana en polvo y leche de una formula comercial a ratones de la cepa Bald/C durante 14 semanas. Se midieron el nivel de glucosa y el peso. Las heces se recolectaron para verificar la presencia de bacterias lacticas. Los ratones se sacrificaron y se pesaron los intestinos, aislando las bacterias lacticas tanto de los intestinos como de las heces. En las cepas aisladas de la leche humana se evaluo el potencial probiotico analizando su capacidad para inhibir patogenos, resistir distintos pH y temperaturas, adherirse y mostrar hidrofobicidad.Resultados: se identifico la presencia de Lactobacillus fermentum LH01, Lactobacillus rhamnosus LH02, Lactobacullis reuteri LH03 y L. plantarum LH05 en la leche humana en polvo. Todas las cepas mostraron resistencia a los pH bajos, a las sales biliares y a la exposicion a enzimas gastricas, asi como una buena hidrofobicidad y capacidad de autoagregacion. Ademas, no presentaron actividad de hemaglutinacion o hemolisis en un medio de cultivo rico en eritrocitos. Observamos que el consumo de leche humana en polvo evita en los ratones el aumento de peso y el estrenimiento.Conclusiones: despues del secado por aspersion, las cepas con posible potencial probiotico pueden conservarse en la leche materna. El consumo de leche humana en polvo con bacterias probioticas evita el estrenimiento y el aumento de peso en los ratones, en comparacion con los alimentados con leche de una formula comercial. EnglishIntroduction: breast milk (MH) contains nutrients and bioactive compounds for child development, including probiotic bacteria, which contribute to intestinal maturation. This benefit accompanies the individual until adulthood. There are new methods such as spray drying that give this compound a good conservation without loss of microbiota. Objective: the aim of this study was to analyze the viability of lactic acid bacteria isolated from human milk with probiotic potential after the spray drying process, as well as to evaluate the possible adhesion in the colon of mice of the Balb/C strain after feeding them powdered human milk and a commercial formula milk. Method: we isolated and identified the presence of lactic acid bacteria with possible probiotic potential in powdered human milk using the MALDI-TOF MS technique. Powdered human milk and a commercial formula milk were fed to mice of the Bald/C strain for 14 weeks. Glucose level and weight were measured in the mice. The feces were collected to verify the presence of lactic bacteria. The mice were sacrificed and their intestines were weighed, isolating the lactic acid bacteria both from the intestines and from the feces. The strains isolated from mice fed human milk were evaluated for their probiotic potential, analyzing their ability to inhibit pathogens, resistance to pH, temperature, adhesion, and hydrophobicity. Results: the presence of Lactobacillus fermentum LH01, Lactobacillus rhamnosus LH02, Lactobacullis reuteri LH03, and Lactobacillus plantarum LH05 in powdered human milk was identified. All strains showed a possible probiotic profile due to the ability of bacteria to resist low pH, bile salts, and exposure to gastric enzymes, as well as their hydrophobicity and self-aggregation capacity, and their failure to show hemagglutination or hemolysis activity in a culture medium rich in erythrocytes. We observed that the consumption of powdered human milk prevented weight gain and constipation in mice. Conclusions: after spray drying, strains with possible probiotic potential may be preserved in human milk. The consumption of powdered human milk with probiotic bacteria prevents constipation and weight gain in mice, when compared to those fed a commercial formula milk. |
| Databáze: | OpenAIRE |
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