Potyvirus Helper Component-Proteinase Self-Interaction in the Yeast Two-Hybrid System and Delineation of the Interaction Domain Involved
| Autor: | Olivier Le Gall, Thierry Candresse, Françoise Bernardi, Silvio Urcuqui-Inchima, Sylvie German-Retana, Gabrièle Drugeon, Jocelyne Walter, Anne-Lise Haenni |
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| Přispěvatelé: | Unité Mixte de Recherche en Santé Végétale (INRA/ENITA) (UMRSV), Institut National de la Recherche Agronomique (INRA)-École Nationale d'Ingénieurs des Travaux Agricoles - Bordeaux (ENITAB)-Institut des Sciences de la Vigne et du Vin (ISVV) |
| Rok vydání: | 1999 |
| Předmět: |
0106 biological sciences
[SDV]Life Sciences [q-bio] Two-hybrid screening nicotiana tabacum Heterologous Saccharomyces cerevisiae Biology gcmv 01 natural sciences Genome Viral Proteins 03 medical and health sciences potyvirus Virology Cloning Molecular double hybride ComputingMilieux_MISCELLANEOUS 030304 developmental biology chemistry.chemical_classification Genetics 0303 health sciences Binding Sites génome Potyvirus dimer biology.organism_classification VIRUS DE LA MOSAIQUE JAUNE-CHROME DE A VIGNE Lettuce mosaic virus Yeast VIROLOGIE Amino acid tabac INSECTE Cysteine Endopeptidases chemistry Potato virus Y HC-PRO plante stimulante 010606 plant biology & botany |
| Zdroj: | Virology 1 (258), 95-99. (1999) Virology Virology, Elsevier, 1999, 258 (1), pp.95-99 |
| ISSN: | 0042-6822 1096-0341 |
| DOI: | 10.1006/viro.1999.9725 |
| Popis: | Using the yeast two-hybrid system, a screen was performed for possible interactions between the proteins encoded by the 5′ region of potyviral genomes [P1, helper component-proteinase (HC-Pro), and P3]. A positive self-interaction involving HC-Pro was detected with lettuce mosaic virus (LMV) and potato virus Y (PVY). The possibility of heterologous interaction between the HC-Pro of LMV and of PVY was also demonstrated. No interaction involving either the P1 or the P3 proteins was detected. A series of ordered deletions from either the N- or C-terminal end of the LMV HC-Pro was used to map the domain involved in interaction to the 72 N-terminal amino acids of the protein, a region known to be dispensable for virus viability but necessary for aphid transmission. A similar but less detailed analysis mapped the interacting domain to the N-terminal half of the PVY HC-Pro. |
| Databáze: | OpenAIRE |
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