| Autor: |
Ming Wang, Zhenhui Zhong, Javier Gallego-Bartolomé, Zheng Li, Suhua Feng, Hsuan Yu Kuo, Ryan L. Kan, Hoiyan Lam, John Curtis Richey, Linli Tang, Jessica Zhou, Mukun Liu, Yasaman Jami-Alahmadi, James Wohlschlegel, Steven E. Jacobsen |
| Rok vydání: |
2023 |
| Předmět: |
|
| Zdroj: |
Nature plants, vol 9, iss 3 |
| ISSN: |
2055-0278 |
| DOI: |
10.1038/s41477-023-01362-8 |
| Popis: |
DNA methylation has been utilized for target gene silencing in plants. However, it is not well understood whether other silencing pathways can be also used to manipulate gene expression. Here we performed a gain-of-function screen for proteins that could silence a target gene when fused to an artificial zinc finger. We uncovered many proteins that suppressed gene expression through DNA methylation, histone H3K27me3 deposition, H3K4me3 demethylation, histone deacetylation, inhibition of RNA polymerase II transcription elongation or Ser-5 dephosphorylation. These proteins also silenced many other genes with different efficacies, and a machine learning model could accurately predict the efficacy of each silencer on the basis of various chromatin features of the target loci. Furthermore, some proteins were also able to target gene silencing when used in a dCas9-SunTag system. These results provide a more comprehensive understanding of epigenetic regulatory pathways in plants and provide an armament of tools for targeted gene manipulation. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
|
Full text from SpringerLink