PERFORMANCE EVALUATION OF NUCLEATED RED BLOOD CELL (NRBC) COUNT USING A FULLY AUTOMATED HAEMATOLOGY ANALYZER VERSUS MANUAL COUNTING
| Autor: | Usman Tahir Swati, Tanweer Ahmed, Nasir Uddin, Helen Mary Robert, Muhammad Ashraf, Asad Mahmood |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
bland-altman plot
medicine.medical_specialty Spectrum analyzer Medicine (General) Hematology business.industry Nucleated Red Blood Cell nucleated rbc count Peripheral blood R5-920 Fully automated Internal medicine Linear regression automated haematology analyzer medicine Medicine Bland–Altman plot Nuclear medicine business Whole blood |
| Zdroj: | Pakistan Armed Forces Medical Journal, Vol 71, Iss 5, Pp 1806-1810 (2021) |
| ISSN: | 2411-8842 0030-9648 |
| Popis: | Objective: To evaluate the performance of Nucleated RBC (NRBC) Count using a fully automated haematology analyzer versus manual counting. Study Design: Cross-Sectional Study. Place and Duration of Study: Department of Hematology, Armed Forces Institute of Pathology, from Sep 2019-Jun 2020. Methodology: Routine fresh whole blood samples were run on Sysmex XN-3000 automated haematology analyzer and 384 samples with results of ≥0.1% Nucleated red blood cells were included in this study. Manual NRBC counting was carried out twice on Leishman-stained peripheral blood smears from all 384 samples. Comparison between manual and automated nucleated red blood cell counting methods was statistically analyzed through linear regression analysis & coefficient correlation. The degree of agreement between two methods was analyzed through Bland-Altman plot. Finally, concordance between the two methods was also analyzed at 5 different ranges of nucleated red blood cells. Results: Linear regression analysis revealed a (r2) value of 0.97. Regression equation was calculated as XN = 0.76MC ± 1.28, with 95% limits of agreement between ± 40.42% and -24.47%. A mean bias of 7.97% was demonstrated through Bland-Altman plot. Concordance analysis revealed a concordance rate of 93.74% (360/384). Nucleated red blood cell counting between two methods were more concordant when nucleated red blood cell counts were |
| Databáze: | OpenAIRE |
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