SV40 Small T Antigen Enhances Progression to >G2 during Lytic Infection
| Autor: | Judith Laffin, John M. Lehman, Thomas D. Friedrich, Brian Whalen |
|---|---|
| Rok vydání: | 1999 |
| Předmět: |
G2 Phase
Antigens Polyomavirus Transforming Blotting Western Cell Cycle Proteins Simian virus 40 Biology Virus Replication Retinoblastoma Protein Virus Cell Line Polyploidy Cyclin D1 Chlorocebus aethiops Animals Mimosine Cytotoxic T cell Phosphorylation Mitosis Cell Nucleus CD40 DNA synthesis Tumor Suppressor Proteins Cell Cycle DNA Cell Biology Molecular biology Kinetics Gene Expression Regulation Lytic cycle Viral replication Mutation biology.protein Microtubule-Associated Proteins Cyclin-Dependent Kinase Inhibitor p27 |
| Zdroj: | Experimental Cell Research. 251:121-127 |
| ISSN: | 0014-4827 |
| DOI: | 10.1006/excr.1999.4572 |
| Popis: | The infection of monkey kidney (CV-1) cells with simian virus 40 (SV40) stimulates the cells into successive rounds of DNA synthesis without an intervening mitosis, leading to the acquisition of a >G2 DNA content. To elucidate the role of small t antigen in cell cycle progression and in viral replication during infection, studies were performed using an SV40 mutant (dl888) that lacks the ability to produce small t. Initially dl888-infected cells move through the first S phase at roughly the same rate as wild-type infected cells. Upon reaching G2, however, the dl888-infected cells progressed to >G2 at a reduced rate relative to wild-type. The slower rate of entry into >G2 of dl888-infected cells is associated with a decrease in total pRb and an increase in the ratio of hypophosphorylated to hyperphosphorylated pRb. The expression of cyclin D1 and p27kip1 were elevated in dl888-infected cells compared to wild-type-infected CV-1 cells. Taken together, these results indicate that small t antigen plays a role in stimulating entry into >G2 in SV40-infected CV-1 cells, possibly by affecting the regulation of key cell cycle proteins. |
| Databáze: | OpenAIRE |
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