Selective hyperexpression of c-jun oncoprotein by glass fiber- and silica-transformed BALB/c-3T3 cells
| Autor: | Huilan Gao, Shu-hui Ong, Wen-Zong Whong, Tong-man Ong, James E. Brick, Michael R. Miller |
|---|---|
| Rok vydání: | 1997 |
| Předmět: |
Cancer Research
Tumor suppressor gene Proto-Oncogene Proteins c-jun Glass fiber Cell Genes myc Biology Malignant transformation Mice Genes jun Gene expression medicine Animals Mice Inbred BALB C c-jun 3T3 Cells Oncogenes Genes p53 Silicon Dioxide Molecular biology In vitro medicine.anatomical_structure Cell Transformation Neoplastic Genes ras Oncology Gene Expression Regulation Polyclonal antibodies biology.protein Glass |
| Zdroj: | Cancer letters. 112(1) |
| ISSN: | 0304-3835 |
| Popis: | Mining and mineral processing are important industries in the United States. A large number of workers are potentially exposed to silica during mining and to glass fibers during manufacturing. There is a concern regarding lung cancer risk among workers exposed to silica and glass fibers. Our previous studies showed that both glass fibers and silica induced transformation of BALB/c-3T3 cells. In order to explore the relationship between silica and glass fiber-induced cell transformation and oncoprotein expression, the protein products of seven proto-oncogenes (c-K-ras, c-H-ras, c-sis, c-myc, c-myb, c-erb B1 and c-jun) and one tumor suppressor gene (p53) were examined in BALB/c-3T3 cells transformed by glass fibers or silica using immunoblotting with specific monoclonal or polyclonal antibodies. The results showed that all transformants, including eight induced by glass fibers and eight by silica (Min-U-Sil 5), were positive for c-jun protein expression; the level of c-jun protein was elevated 8-21-fold in these transformants. Other protooncogene proteins in transformed cells were either not detectable or not different from non-transformed cells. These results suggest that the overexpression of c-jun is common in BALB/c-3T3 transformed cells induced by glass fibers or silica. It seems, therefore, that the expression of c-jun may play an important role in the transformation process. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect