Microbial community profiling of ammonia and nitrite oxidizing bacterial enrichments from brackishwater ecosystems for mitigating nitrogen species
| Autor: | M. Leo Antony, Sudeep D. Ghate, Shankar Vinayakarao Alavandi, Suganya Nathamuni, Prasanna Kumar Patil, Satheesha Avunje, N. Dineshkumar, Viswanathan Baskaran, Kizhakedath K. Vijayan, Vinay Tharabenahalli Nagaraju |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Denitrification 030106 microbiology Microbial metabolism Nitrosopumilus lcsh:Medicine Ribotyping Article 03 medical and health sciences Species Specificity Ammonia Element cycles lcsh:Science Saline Waters Nitrogen cycle Nitrites Nitrosomonas Autotrophic Processes Multidisciplinary Bacteria biology Chemistry Microbiota lcsh:R Heterotrophic Processes Nitrogen Cycle biology.organism_classification Nitrification 030104 developmental biology Microbial population biology Nitrifying bacteria Environmental chemistry Metagenome lcsh:Q Metagenomics Water Microbiology Oxidation-Reduction Metabolic Networks and Pathways |
| Zdroj: | Scientific Reports, Vol 10, Iss 1, Pp 1-11 (2020) Scientific Reports |
| ISSN: | 2045-2322 |
| DOI: | 10.1038/s41598-020-62183-9 |
| Popis: | Nitrogen species such as ammonia and nitrite are considered as major stressors in modern aquaculture practices. We developed enrichments of ammonia oxidising bacteria (AOB) and nitrite oxidising bacteria (NOB) for effective mitigation of nitrogenous wastes in the shrimp culture operations. The objective of this study was to understand the microbial community composition of AOB and NOB enrichments using the V3-V4 region of the 16S rDNA gene by Illumina MiSeq sequencing. The analysis revealed 2948 and 1069 OTUs at 97% similarity index and Shannon alpha diversity index of 7.64 and 4.85 for AOB and NOB enrichments, respectively. Comparative analysis showed that a total of 887 OTUs were common among AOB and NOB enrichments. The AOB and NOB enrichment were dominated by Eubacteria at 96% and 99.7% respectively. Proteobacterial phylum constituted 31.46% (AOB) and 39.75% (NOB) and dominated by α-Proteobacteria (20%) in AOB and γ-Proteobacteria (16%) in NOB. Among the species in AOB enrichment (2,948) two sequences were assigned to ammonia oxidising bacterial group belonging to Nitrosomonas, and Nitrosococcus genera and two belonged to archaeon group comprising Nitrosopumilus and Candidatus Nitrososphaeraea genera. The NOB enrichment was predominated by Nitrospiraceae and Thermodesulfovibrionaceae. Further, the data revealed the presence of heterotrophic bacteria contributing to the process of nitrification and form microcosm with the AOB and NOB. PICRUSt analysis predicted the presence of 24 different nitrogen cycling genes involved in nitrification, denitrification, ammonia and nitrogen transporter family, nitrate reduction and ammonia assimilation. The study confirms the presence of many lesser known nitrifying bacteria along with well characterised nitrifiers. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|