T-2 toxin inhibits the differentiation of human monocytes into dendritic cells and macrophages
| Autor: | J.-L. Jung, N. Hymery, François-Gilles Carpentier, K. Léon, Dominique Parent-Massin |
|---|---|
| Přispěvatelé: | Laboratoire de Biologie et génétique des mammifères marins dans leur environnement (BioGEMME), Université de Brest (UBO) |
| Jazyk: | angličtina |
| Rok vydání: | 2009 |
| Předmět: |
Cell Survival
CD14 Down-Regulation 010501 environmental sciences Biology Toxicology [SDV.BID.SPT]Life Sciences [q-bio]/Biodiversity/Systematics Phylogenetics and taxonomy 01 natural sciences Monocytes 03 medical and health sciences Phagocytosis Antigens CD medicine Humans Macrophage Cytotoxic T cell ComputingMilieux_MISCELLANEOUS Respiratory Burst 030304 developmental biology 0105 earth and related environmental sciences 0303 health sciences CLEC7A Macrophages Monocyte Infant Newborn Cell Differentiation Dendritic Cells General Medicine Dendritic cell Fetal Blood Flow Cytometry Endocytosis Cell biology T-2 Toxin medicine.anatomical_structure Monocyte differentiation Biomarkers CD80 |
| Zdroj: | Toxicology in Vitro Toxicology in Vitro, Elsevier, 2009, 23 (3), pp.509-519. ⟨10.1016/j.tiv.2009.01.003⟩ |
| ISSN: | 0887-2333 |
| DOI: | 10.1016/j.tiv.2009.01.003⟩ |
| Popis: | The aim of this work was to study the in vitro effect of T-2 toxin on human monocyte differentiation into macrophages and dendritic cells. Cytotoxicity of T-2 toxin on monocytes, on monocytes in differentiation process into macrophages or dendritic cells, and on immature dendritic cells and macrophages was evaluated to determine IC50. Monocytes are more sensitive to T-2 toxin than to differentiate cells. IC50 were equal to 0.11 nM for monocyte, to 45 and 30 nM for monocyte during differentiation process for 24 and 48 h of incubation, respectively, to 38 and 20 nM for immature dendritic cells after 24 and 48 h of incubation, and to 22 and 20 nM for macrophages after 24 and 48 h of incubation. T-2 toxin effects on monocyte differentiation process into macrophages have been explored: according to phenotypic expressions (CD71, CD14, CD11a, CD80, CD86, HLA-DR and CD64), endocytic capacity, phagocytosis, burst respiratory activity and TNF-alpha secretion. In the presence of 10 nM of T-2 toxin (no cytotoxic concentration), CD71 expression is downregulated compared to control. Endocytosis and phagocytosis capacities are less effective as burst respiratory activity and TNF-alpha secretion. Monocyte differentiation process into dendritic cells in the presence of 10 nM T-2 toxin is also markedly disturbed. Expression of CD1a (specific dendritic cells marker) is downregulated while that of CD14 (specific monocyte marker) is upregulated. CD11a, CD80, CD86, HLA-DR and CD64 expressions did not change. These results show that T-2 toxin disturbs human monocytes differentiation process into macrophages and dendritic cells. These results could significantly contribute to immunosuppressive properties of this alimentary toxin. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect