Yield and proliferation rate of adipose-derived stromal cells as a function of age, body mass index and harvest site-increasing the yield by use of adherent and supernatant fractions?
| Autor: | Johanna Buschmann, Manfred Welti, Sonja Hemmi, Maurizio Calcagni, Shuping Gao, Luc Härter, Guido A. Wanner, Paolo Cinelli, Clément M. L. Werner |
|---|---|
| Přispěvatelé: | University of Zurich, Buschmann, Johanna |
| Rok vydání: | 2012 |
| Předmět: |
Adult
Male 2716 Genetics (clinical) Cancer Research Stromal cell 2747 Transplantation Immunology CD34 Cell Culture Techniques Adipose tissue 610 Medicine & health Flow cytometry Body Mass Index 1307 Cell Biology Andrology Antigens CD medicine Immunology and Allergy Doubling time Humans 1306 Cancer Research 10266 Clinic for Reconstructive Surgery Genetics (clinical) Cells Cultured Aged Cell Proliferation 2403 Immunology Transplantation medicine.diagnostic_test biology Chemistry Stem Cells Mesenchymal stem cell CD44 Cell Biology Middle Aged 10021 Department of Trauma Surgery 10022 Division of Surgical Research Oncology Adipose Tissue 2723 Immunology and Allergy biology.protein 2730 Oncology Female Stem cell |
| Zdroj: | Cytotherapy. 15(9) |
| ISSN: | 1477-2566 |
| Popis: | Background aims. Adipose-derived stem cells are easily accessed and have a relatively high density compared with other mesenchymal stromal cells. Isolation protocols of adipose-derived stem cells (ASC) rely on the cell’s ability to adhere to tissue culture plastic overnight. It was evaluated whether the floating ASC fractions are also of interest for cell-based therapies. In addition, the impact of age, body mass index (BMI) and harvest site was assessed. Methods. The surface protein profile with the use of flow cytometry, the cell yield and the doubling time of passages 4, 5 and 6 of ASC from 30 donors were determined. Adherent and supernatant fractions were compared. The impact of age, BMI and harvest site on cell yield and doubling times was determined. Results. Both adherent and supernatant fractions showed high mean fluorescence intensities for CD13, CD29, CD44, CD73, CD90 and CD105 and comparatively low mean fluorescence intensities for CD11b, CD62L, intracellular adhesion molecule-1 and CD34. Doubling times of adherent and supernatant fractions did not differ significantly. Whereas the old age group had a significantly lower cell yield compared with the middle aged group, BMI and harvest site had no impact on cell yield. Finally, doubling times for passages 4, 5 and 6 were not influenced by the age and BMI of the donors, nor the tissue-harvesting site. Conclusions. The floating ASC fraction is an equivalent second cell source just like the adherent ASC fraction. Donor age, BMI and harvest site do not influence cell yield and proliferation rate. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect