Rourea cuspidata : Chemical composition and hypoglycemic activity
| Autor: | Manuela M. Laikowski, Paulo Roberto dos Santos, Gisiele Alano, Mariana Roesch-Ely, Leandro Tasso, Debora M. de Souza, Luciane Minetto, Sidnei Moura, Natalia Girondi, Camila Cervi Pires |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
lcsh:Arctic medicine. Tropical medicine Cuspidata lcsh:RC955-962 HRMS Pharmacology 01 natural sciences Biochemistry Genetics and Molecular Biology (miscellaneous) Glibenclamide 03 medical and health sciences Drug control Oral administration Rourea cuspidata Diabetes mellitus medicine lcsh:QH301-705.5 Alanine biology 010405 organic chemistry Chemistry Diabetes Phytochemical characterization medicine.disease biology.organism_classification 0104 chemical sciences 030104 developmental biology medicine.anatomical_structure lcsh:Biology (General) Biochemistry Active compound Pancreas medicine.drug |
| Zdroj: | Asian Pacific Journal of Tropical Biomedicine, Vol 7, Iss 8, Pp 712-718 (2017) |
| ISSN: | 2221-1691 |
| DOI: | 10.1016/j.apjtb.2017.07.015 |
| Popis: | Objective To investigate the antidiabetic effect of Rourea cuspidata hydroalcoholic stem extract in normal and streptozotocin-induced diabetic rats. Methods In order to evaluate the chemical composition, different extracts from stem in ascending solvent order of polarity were prepared. The extracts were analyzed by high resolution mass spectrometry and 7 compounds were identified, including hyperin, an important and already reported active compound in the literature. Hyperin was also quantified by HPLC-UV in all the extracts. The hydroalcoholic stem extract (Ss5), which showed the highest concentration of hyperin, was administered to STZ-induced diabetes rats to evaluate the potential hypoglycemic activity. Total cholesterol, HDL, triglycerides, ALT and AST were also evaluated. In the present study, the effects of oral administration of hydroalcoholic stem extract (200 mg/kg b. wt.) for 28 days on the level of serum glucose, total cholesterol, HDL, triglycerides, aspartate amino transferase (AST) and alanine amino transferase (ALT) in normal and streptozotocin-induced diabetic rats were evaluated. Histopathological changes in diabetic rats' pancreas were also studied. Results The extract exposition demonstrated hypoglycemic effect like the drug control glibenclamide. The extract was able to increase the HDL levels. Histopathological study on diabetic rats' pancreas after extract treatment showed morphological alterations in STZ-induced diabetes rats, which were apparently restored after extract treatment. Conclusions This work demonstrates the potential use of R. cuspidata as hypoglycemic agent in the treatment of diabetes. |
| Databáze: | OpenAIRE |
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