Biologic activity of guinea pig IFN-gamma in vitro
| Autor: | Reinhard Burger, Petra Meuer, Albrecht F. Kiderlen, Hubert Schäfer, Katrin Klippert, Bettina Borsdorf |
|---|---|
| Rok vydání: | 2007 |
| Předmět: |
Immunology
Guinea Pigs Cavia Response Elements Antiviral Agents law.invention Guinea pig Interferon-gamma Mice law Genes Reporter Virology Animals Humans Cells Cultured Cytopathic effect Reporter gene biology Cell Biology Transfection Fibroblasts biology.organism_classification Molecular biology In vitro Recombinant Proteins Virus Diseases Recombinant DNA Rabbits Intracellular |
| Zdroj: | Journal of interferoncytokine research : the official journal of the International Society for Interferon and Cytokine Research. 27(4) |
| ISSN: | 1079-9907 |
| Popis: | Interferon-gamma (IFN-gamma) plays a key role in the induction and maintenance of immunity against intracellular infectious agents. Compared to other species, little is known about the biology of this cytokine in the guinea pig (Cavia porcellus). We found that in contrast to humans and mice, IFN-gamma in the guinea pig did not induce the antiviral state, which in other species leads to protection of IFN-gamma -stimulated fibroblasts from the cytopathic effect (CPE) of subsequent viral infections. As an alternative strategy to detect and quantify guinea pig IFN-gamma activity in vitro, a reporter system using guinea pig fibroblasts transfected with a luciferase gene, which is regulated by an IFN-stimulated response element (ISRE), was established. With the help of the highly sensitive reporter assay system, the biologic activity of recombinant guinea pig IFN-gamma (GpIFN-gamma, from prokaryotic and eukaryotic expression systems was detected. The response to both native and recombinant GpIFN-gamma was inhibited by a rabbit antiserum directed against the recombinant cytokine expressed in Escherichia coli, demonstrating structural and functional homology of native and recombinant GpIFN-gamma. Stimulation with GpIFN-gamma, obtained from transfected cells, induced upregulation of MHC class I expression in a guinea pig fibroblast line. The restricted activity of GpIFN-gamma might have implications for this species' ability to control infections with intracellular pathogens. |
| Databáze: | OpenAIRE |
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