Two Kdo-Heptose Regions Identified inHafnia alvei32 Lipopolysaccharide: the Complete Core Structure and Serological Screening of DifferentHafniaO Serotypes
Autor: | Wojciech Jachymek, Tomasz Niedziela, Czeslaw Lugowski, Jolanta Lukasiewicz, Lennart Kenne |
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Rok vydání: | 2009 |
Předmět: |
Lipopolysaccharides
Magnetic Resonance Spectroscopy Gram-negative bacteria Molecular Sequence Data Heptose Microbiology Structural Biology Animals Humans Trisaccharide Serotyping Molecular Biology chemistry.chemical_classification biology Enterobacteriaceae Infections O Antigens Sugar Acids Hafnia alvei Nuclear magnetic resonance spectroscopy Oligosaccharide Heptoses biology.organism_classification Hafnia Enterobacteriaceae Carbohydrate Sequence chemistry lipids (amino acids peptides and proteins) Rabbits Trisaccharides Bacteria |
Zdroj: | Journal of Bacteriology. 191:533-544 |
ISSN: | 1098-5530 0021-9193 |
DOI: | 10.1128/jb.00891-08 |
Popis: | Hafnia alvei, a gram-negative bacterium, is an opportunistic pathogen associated with mixed hospital infections, bacteremia, septicemia, and respiratory diseases. Various 3-deoxy-d-manno-oct-2-ulosonic acid (Kdo)-containing fragments different from known structures of core oligosaccharides were previously found among fractions obtained by mild acid hydrolysis of someH. alveilipopolysaccharides (LPSs). However, the positions of these segments in the LPS structure were not known. Analysis of de-N,O-acylated LPS by nuclear magnetic resonance spectroscopy and mass spectrometry allowed the determination of the location of a Kdo-containing trisaccharide in the structure ofH. alveiPCM 32 LPS. It was established that the trisaccharide {l-α-d-Hepp-(1→4)-[α-d-Galp6OAc-(1→7)]-α-Kdop-(2→} is an integral part of the outer-core oligosaccharide ofH. alvei32 LPS. The very labile ketosidic linkage between →4,7)-α-Kdopand →2)-Glcpin the core oligosaccharide was identified. Screening for this Kdo-containing trisaccharide was performed on the group of 37 O serotypes ofH. alveiLPSs using monospecific antibodies recognizing the structure. It was established that this trisaccharide is a characteristic component of the outer-core oligosaccharides ofH. alvei2, 32, 600, 1192, 1206, and 1211 LPSs. The weaker cross-reactions with LPSs of strains 974, 1188, 1198, 1204, and 1214 suggest the presence of similar structures in these LPSs, as well. Thus, we have identified new examples of endotoxins among those elucidated so far. This type of core oligosaccharide deviates from the classical scheme by the presence of the structural Kdo-containing motif in the outer-core region. |
Databáze: | OpenAIRE |
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