Charging the Quantum Capacitance of Graphene with a Single Biological Ion Channel
Autor: | Peter Burke, Jinfeng Li, Yung Yu Wang, Katayoun Zand, Ted Pham |
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Rok vydání: | 2014 |
Předmět: |
Lipid Bilayers
General Physics and Astronomy Nanotechnology Biosensing Techniques Electrolyte Electric Capacitance Spectrum Analysis Raman biosensor Ion Channels Article Membrane Potentials law.invention Electrolytes Quantum capacitance law Animals Humans General Materials Science Alamethicin Lipid bilayer Ion channel Ions Graphene Chemistry graphene Gramicidin General Engineering Hydrogen-Ion Concentration Lipids lipid bilayer Electrophysiology Membrane Nanoelectronics ion channel Solvents Quantum Theory transistor Graphite |
Zdroj: | ACS Nano |
ISSN: | 1936-086X 1936-0851 |
Popis: | The interaction of cell and organelle membranes (lipid bilayers) with nanoelectronics can enable new technologies to sense and measure electrophysiology in qualitatively new ways. To date, a variety of sensing devices have been demonstrated to measure membrane currents through macroscopic numbers of ion channels. However, nanoelectronic based sensing of single ion channel currents has been a challenge. Here, we report graphene-based field-effect transistors combined with supported lipid bilayers as a platform for measuring, for the first time, individual ion channel activity. We show that the supported lipid bilayers uniformly coat the single layer graphene surface, acting as a biomimetic barrier that insulates (both electrically and chemically) the graphene from the electrolyte environment. Upon introduction of pore-forming membrane proteins such as alamethicin and gramicidin A, current pulses are observed through the lipid bilayers from the graphene to the electrolyte, which charge the quantum capacitance of the graphene. This approach combines nanotechnology with electrophysiology to demonstrate qualitatively new ways of measuring ion channel currents. |
Databáze: | OpenAIRE |
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