Quantification of cystatin C in cerebrospinal fluid from various neurological disorders and correlation with G73A polymorphism in CST3
| Autor: | Hiroyuki Akimoto, Etsuro Matsubara, Mikio Shoji, Kazuhiro Sugimoto, Yasuhito Wakasaya, Mandy Jackson, Mitsunori Watanabe, Yukiko Yamamoto-Watanabe, Minoru Yasujima, Alastair Robert Lyndon, Takeshi Kawarabayashi, Yasuo Harigaya |
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| Rok vydání: | 2010 |
| Předmět: |
Male
Pathology medicine.medical_specialty Latex Glycine Chronic inflammatory demyelinating polyneuropathy Neurological disorder Polymorphism Single Nucleotide Central nervous system disease Atrophy Cerebrospinal fluid Alzheimer Disease Nephelometry and Turbidimetry medicine Humans Cystatin C Amyotrophic lateral sclerosis Molecular Biology Aged Aged 80 and over Immunoassay Alanine biology business.industry General Neuroscience Amyotrophic Lateral Sclerosis Parkinson Disease Middle Aged Multiple System Atrophy medicine.disease Polyradiculoneuropathy Chronic Inflammatory Demyelinating Tauopathies Case-Control Studies biology.protein Female Neurology (clinical) Cystatin Nervous System Diseases business Biomarkers Developmental Biology |
| Zdroj: | Brain Research. 1361:140-145 |
| ISSN: | 0006-8993 |
| DOI: | 10.1016/j.brainres.2010.09.033 |
| Popis: | Cystatin C (CC) is a cysteine protease inhibitor abundantly expressed in the central nervous system. Bunina bodies, small eosinophilic intraneuronal inclusions, are stain positive for CC and are the most specific histological hallmark of amyotrophic lateral sclerosis (ALS). In this study, employing a latex turbidimetric immunoassay, levels of CC in cerebrospinal fluid (CSF) were quantified in 130 age-matched individuals with either a neurological disorder [ALS, Alzheimer's disease (AD), Parkinson's disease (PD), tauopathy (TP), multiple system atrophy (MSA), chronic inflammatory demyelinating polyneuropathy (CIDP)] or no known neurological condition (normal control, NC). The CC level in CSF was found to be correlated with the age during the investigation but not the protein concentration. There was no difference in CC levels between NC and ALS or CIDP cases, whereas CC levels were significantly lower in MSA compared with NC. Of the 130 cases, 96 were genotyped, and G/A or A/A polymorphism at +73 within the CST3 gene was found in 28 individuals. The CC level was significantly lower in the combined group of G/A and A/A genotypes compared with G/G. The present data demonstrate that the level of CC in CSF should not be considered as a biomarker of ALS, but there is a correlation between CC levels and the CST3 genotype. |
| Databáze: | OpenAIRE |
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