Bioorthogonally Applicable Fluorogenic Cyanine-Tetrazines for No-Wash Super-Resolution Imaging
| Autor: | György Török, Eszter Kozma, Gergely Knorr, Péter Kele, Krisztina Németh, Judith M. Schaart |
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| Rok vydání: | 2018 |
| Předmět: |
Biomedical Engineering
Pharmaceutical Science Bioengineering 010402 general chemistry 01 natural sciences Tetrazine chemistry.chemical_compound Heterocyclic Compounds 1-Ring Microscopy Chlorocebus aethiops Animals Cyanine Fluorescent Dyes Pharmacology Microscopy Confocal 010405 organic chemistry Organic Chemistry Optical Imaging STED microscopy Carbocyanines Superresolution Fluorescence 0104 chemical sciences Fluorescence intensity Actin Cytoskeleton Spectrometry Fluorescence chemistry COS Cells Biophysics Bioorthogonal chemistry Biotechnology |
| Zdroj: | Bioconjugate chemistry. 29(4) |
| ISSN: | 1520-4812 |
| Popis: | The synthesis, fluorogenic characterization, and labeling application of four tetrazine-quenched cyanine probes with emission maxima in the red–far red range is reported. Fluorescence of the cyanine-cores is quenched via through-bond-energy-transfer (TBET) exerted by a bioorthogonal tetrazine unit. Upon bioorthogonal labeling reaction with cyclooctyne tagged proteins, the quenching effect ceases, and thus the fluorescence reinstates, resulting in an increase in fluorescence intensity. As a rare example among indocyanines, one of our new probes was found suitable in STED-based super-resolution imaging. The applicability of this fluorogenic Tet-Cy3 probe was therefore further demonstrated in the bioorthogonal labeling of cytoskeletal protein, actin, with subsequent super-resolution microscopy (STED) imaging even under no-wash conditions. |
| Databáze: | OpenAIRE |
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