Burden and genotype distribution of high-risk Human Papillomavirus infection and cervical cytology abnormalities at selected obstetrics and gynecology clinics of Addis Ababa, Ethiopia

Autor: Mesfin Nigussie Difabachew, Zufan Lakew Woldu, Eshetu Lemma Haile, Tsegaye Hailu Kumssa, Ibrahim Mohammed, Dawit Solomon Demeke, Kassu Desta Tullu, Kirubel Eshetu Ali, Robert-Jan ten Hove, Tasew Haile
Jazyk: angličtina
Rok vydání: 2019
Předmět:
0301 basic medicine
Cancer Research
Uterine Cervical Neoplasms
Cervix Uteri
Cohort Studies
0302 clinical medicine
Obstetrics and gynaecology
High-risk Human Papillomavirus
Cytology
HIV Seropositivity
Genotype
Mass Screening
Cervical cytology
Early Detection of Cancer
Cervical cancer
Human papillomavirus 16
Human papillomavirus 18
Obstetrics
Age Factors
Middle Aged
lcsh:Neoplasms. Tumors. Oncology. Including cancer and carcinogens
Oncology
030220 oncology & carcinogenesis
Obstetrics and gynecology
Female
Papanicolaou Test
Research Article
Cohort study
Adult
medicine.medical_specialty
Adolescent
Real-Time Polymerase Chain Reaction
lcsh:RC254-282
Young Adult
03 medical and health sciences
Atypical Squamous Cells of the Cervix
Genetics
medicine
Genotype distribution
Humans
Disease burden
Vaginal Smears
business.industry
Pap cytology
Papillomavirus Infections
Epithelial Cells
medicine.disease
Cross-Sectional Studies
030104 developmental biology
Ethiopia
Gynecological Examination
business
Serostatus
Ascus
Real-time PCR
Zdroj: BMC Cancer, Vol 19, Iss 1, Pp 1-9 (2019)
BMC Cancer
ISSN: 1471-2407
Popis: Background Human papillomavirus is recognized as a major cause of cervical cancer. It is estimated that annually, 7,095 women are diagnosed with cervical cancer and 4,732 die from the disease in Ethiopia. Understanding that the screening practice is very poor and the coverage is very limited, this disease burden is one of the major public health agendas in Ethiopia. This study aimed to assess the burden and genotype distribution of high-risk human papillomavirus (HR HPV) infection and cervical cytology abnormalities at selected obstetrics and gynecology clinics of Addis Ababa, Ethiopia. Methods An institutional-based cross-sectional study design was employed from June to October 2015. Cervical samples were collected from 366 participants based on inclusion criteria. HR HPV DNA was analyzed using an Abbott Real-Time PCR system, and cervical cytology screening was performed using the conventional Pap-smear technique. Data were entered in to Epi-data version 13 and analyzed using STATA version 11. Results The overall HR HPV burden and abnormal cytology were 13.7 and 13.1%, respectively. The majority of HR HPV types were other than types 16 and 18. Of the total abnormal cytology results, 81.3% were low-grade squamous intraepithelial lesions (LSILs), and 12.5 and 6.3% were atypical squamous cells of undetermined significance (ASCUS) and high-grade squamous intraepithelial lesions (HSILs), respectively. Residence, occupation, and HIV serostatus were significantly associated with HR HPV infection. Among the variables, age, age at first marriage, and education were the only ones associated with cervical cytology abnormalities. The overall agreement between the real-time PCR and Pap cytology screening methods was 78.96% (Kappa value of 0.12, 95% CI (0.00–0.243), P = 0.01). Conclusions Non-16/18 HR HPV genotypes represented the largest proportion of HR HPV infections in this study. Women without cervical cytology abnormalities had the highest frequency of HR HPV infection. A large-scale community-based cohort study shall be designed and implemented to further identifying the persistent genotype and assessing the changes in cervical epithelial cell lines. Electronic supplementary material The online version of this article (10.1186/s12885-019-5953-1) contains supplementary material, which is available to authorized users.
Databáze: OpenAIRE
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