Identification of Bacillus Strains for Biological Control of Catfish Pathogens
| Autor: | Chao Ran, Ei Lin Ooi, Jeffery S. Terhune, Nancy K. Capps, Malachi A. Williams, Bui C. T. Dan, Joseph C. Newton, Craig L. Browdy, Joseph W. Kloepper, Mark R. Liles, Abel A. Carrias |
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| Jazyk: | angličtina |
| Rok vydání: | 2012 |
| Předmět: |
Applied Microbiology
Veterinary Microbiology Bacillus Aquaculture Fish Diseases Gram Negative Ictaluridae Spores Bacterial Multidisciplinary Enterobacteriaceae Infections Agriculture Edwardsiella ictaluri Bacterial Pathogens Aeromonas hydrophila Bacterial Typing Techniques Host-Pathogen Interaction Intestines Biological Control Agents Medicine Ichthyology Catfish Research Article animal structures Science Fisheries Marine Biology Biology Microbiology Microbial Control Sepsis Antibiosis Drug Resistance Bacterial Animals Streptococcus iniae Microbial Pathogens Gram Positive Microbial Viability Edwardsiella tarda fungi Fisheries Science biology.organism_classification Animal Feed Flavobacterium columnare Veterinary Science Yersinia ruckeri Gram-Negative Bacterial Infections Zoology |
| Zdroj: | PLoS ONE PLoS ONE, Vol 7, Iss 9, p e45793 (2012) |
| ISSN: | 1932-6203 |
| Popis: | Bacillus strains isolated from soil or channel catfish intestine were screened for their antagonism against Edwardsiella ictaluri and Aeromonas hydrophila, the causative agents of enteric septicemia of catfish (ESC) and motile aeromonad septicaemia (MAS), respectively. Twenty one strains were selected and their antagonistic activity against other aquatic pathogens was also tested. Each of the top 21 strains expressed antagonistic activity against multiple aquatic bacterial pathogens including Edwardsiella tarda, Streptococcus iniae, Yersinia ruckeri, Flavobacterium columnare, and/or the oomycete Saprolegnia ferax. Survival of the 21 Bacillus strains in the intestine of catfish was determined as Bacillus CFU/g of intestinal tissue of catfish after feeding Bacillus spore-supplemented feed for seven days followed by normal feed for three days. Five Bacillus strains that showed good antimicrobial activity and intestinal survival were incorporated into feed in spore form at a dose of 8×10(7) CFU/g and fed to channel catfish for 14 days before they were challenged by E. ictaluri in replicate. Two Bacillus subtilis strains conferred significant benefit in reducing catfish mortality (P |
| Databáze: | OpenAIRE |
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