Photochemical Tissue Bonding: A Promising Technique for Peripheral Nerve Repair
| Autor: | Tuan Nguyen, Pejman M. Motarjem, Anne C. O'Neill, Irene E. Kochevar, Robert W. Redmond, Christopher Amann, Jonathan M. Winograd, T. Shane Johnson, Mark A. Randolph |
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| Rok vydání: | 2007 |
| Předmět: |
Male
medicine.medical_specialty Light Photochemistry Nerve Fibers Myelinated Rats Sprague-Dawley Suture (anatomy) Epineurium Peripheral nerve medicine Animals Peripheral Nerves Muscle Skeletal Wound Healing Sutures business.industry Regeneration (biology) Histology Combined Modality Therapy Rats Surgery Peripheral Cross-Linking Reagents medicine.anatomical_structure Photochemotherapy Cuff Tissue Adhesives Sciatic nerve Sciatic Neuropathy business |
| Zdroj: | Journal of Surgical Research. 143:224-229 |
| ISSN: | 0022-4804 |
| DOI: | 10.1016/j.jss.2007.01.028 |
| Popis: | Background Photochemical tissue bonding (PTB) is a novel tissue repair technique that uses visible light and a photosensitizing dye to crosslink proteins on tissue surfaces. This technique has been successfully demonstrated in a number of tissue repair models. An ideal nerve repair technique would be atraumatic and avoid placement of foreign bodies at the repair site. The epineurium is suited to photochemical repair as it is thin, translucent and has a relatively high collagen content. This study was designed to determine if PTB could be successfully applied in a peripheral nerve repair model. Material and methods Forty Sprague Dawley rats underwent transection of the sciatic nerve. Animals were then randomized to four treatment groups; epineurial suture repair, epineurial cuff with PTB, epineurial cuff alone, and no repair. Functional recovery was assessed at 10 day intervals using walking track analysis and sciatic function index calculations. At 90 days postoperatively animals were sacrificed and sciatic nerves harvested for histology and histomorphometry. Results Functional recovery in the suture repair and epineural cuff with PTB groups were not significantly different (−70.6 ± 17.8 versus −76.9 ± 10.3, P = 0.64) at 90 days postrepair. Histology showed good axonal regeneration with all repair techniques. Histomorphometric analysis found no significant difference between the repair groups. Conclusions This study illustrates that peripheral nerves can be successfully repaired using a photochemical tissue bonding technique with results similar to those achieved with the current gold standard. With further development and refinement PTB may prove a useful tool in peripheral nerve repair. |
| Databáze: | OpenAIRE |
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