Dynamic Expression of Platelet-Derived Growth Factor-D and Phosphorylated Platelet-Derived Growth Factor Receptor-Β after Traumatic Brain Injury in Rats
| Autor: | Wei Zhu, Peng Yang, Yili Yang, Feng Xu, Zhenyu Cai, Haoxin Zhao |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
medicine.medical_specialty
Traumatic brain injury medicine.medical_treatment Immunofluorescence General Biochemistry Genetics and Molecular Biology Receptor Platelet-Derived Growth Factor beta Growth factor receptor Western blot Internal medicine Brain Injuries Traumatic medicine Animals Platelet-Derived Growth Factor medicine.diagnostic_test biology Brain Neoplasms business.industry Growth factor medicine.disease Rats nervous system diseases Endocrinology nervous system biology.protein Phosphorylation business Platelet-derived growth factor receptor PLATELET-DERIVED GROWTH FACTOR D |
| Zdroj: | Clinical Laboratory. 66 |
| ISSN: | 1433-6510 |
| DOI: | 10.7754/clin.lab.2020.200328 |
| Popis: | BACKGROUND We explored the dynamic expression of platelet-derived growth factor-D (PDGF-D) and phosphorylated platelet-derived growth factor receptor-β (p-PDGFR-β) after traumatic brain injury in rats to provide theoretical basis for selecting therapeutic target and intervention time after traumatic brain injury. METHODS This study prepared the weight drop/impact acceleration-induced traumatic brain injury (TBI) model in rats, including sham group and TBI groups at different observation time points (6 hours, 12 hours, 24 hours, 3 days, and 7 days after TBI). The dynamic expression of PDGF-D and p-PDGFR-β after TBI were detected by western blot and immunofluorescence staining. RESULTS The expression level of PDGF-D and p-PDGFR-β after TBI was detected by western blot. The PDGF-D level was increased (p < 0.05) 6 hours after TBI and remained at the high level until 3 days after TBI (p < 0.05). The p-PDGFR-β level was increased (p < 0.05) 12 hours after TBI and remained at the high level until 3 days after TBI (p < 0.05). PDGF-D and p-PDGFR-β in brain tissues were found by immunofluorescence in the perihematoma area 24 hours after TBI. CONCLUSIONS This study revealed the expression phenomenon of PDGF-D and p-PDGFR-β after TBI in rats, suggesting that PDGF-D participates in the process of secondary brain injury after TBI through specific binding with PDGFR-β, which provides a theoretical basis for further research on selecting therapeutic targets and intervention times after TBI. |
| Databáze: | OpenAIRE |
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