Single step nanoplasmonic immunoassay for the measurement of protein biomarkers
| Autor: | Amit S. Paranjape, Richard M. Awdeh, Adam de la Zerda, Shradha Prabhulkar |
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| Rok vydání: | 2013 |
| Předmět: |
Analyte
glucose transporter-17 Calibration curve lcsh:Biotechnology Clinical Biochemistry Microfluidics glucose transporter-1 Nanotechnology 02 engineering and technology 010402 general chemistry 01 natural sciences glucose transporter-17 Light scattering Article lcsh:TP248.13-248.65 medicine immunoassay Surface plasmon resonance Chromatography optical coherence tomography medicine.diagnostic_test Chemistry protein biomarkers General Medicine 021001 nanoscience & nanotechnology gold nanorods 0104 chemical sciences Immunoassay Nanorod 0210 nano-technology Biosensor surface plasmon resonance |
| Zdroj: | Biosensors Biosensors, Vol 3, Iss 1, Pp 77-88 (2013) Volume 3 Issue 1 Pages: 77-88 |
| ISSN: | 2079-6374 |
| Popis: | A nanoplasmonic biosensor for highly-sensitive, single-step detection of protein biomarkers is presented. The principle is based on the utilization of the optical scattering properties of gold nanorods (GNRs) conjugated to bio-recognition molecules. The nanoplasmonic properties of the GNRs were utilized to detect proteins using near-infrared light interferometry. We show that the antibody-conjugated GNRs can specifically bind to our model analyte, Glucose Transporter-1 (Glut-1). The signal intensity of back-scattered light from the GNRs bound after incubation, correlated well to the Glut-1 concentration as per the calibration curve. The detection range using this nanoplasmonic immunoassay ranges from 10 ng/mL to 1 ug/mL for Glut-1. The minimal detectable concentration based on the lowest discernable concentration from zero is 10 ng/mL. This nanoplasmonic immunoassay can act as a simple, selective, sensitive strategy for effective disease diagnosis. It offers advantages such as wide detection range, increased speed of analysis (due to fewer incubation/washing steps), and no label development as compared to traditional immunoassay techniques. Our future goal is to incorporate this detection strategy onto a microfluidic platform to be used as a point-of-care diagnostic tool. |
| Databáze: | OpenAIRE |
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