Evaluation of the Protective Effect of Silibinin in Rats with Liver Damage Caused by Itraconazole
| Autor: | Esin Sakalli Cetin, Halil Beydilli, Alper Aksozek, Özgür İlhan Çelik, Hamdi Sözen, Nigar Yilmaz, Yaşar Topal, İbrahim Hakkı Ciğerci |
|---|---|
| Rok vydání: | 2014 |
| Předmět: |
Antioxidant
Itraconazole DNA damage medicine.medical_treatment Biophysics Silibinin Pharmacology medicine.disease_cause Biochemistry Antioxidants chemistry.chemical_compound medicine Animals Drug Interactions Rats Wistar chemistry.chemical_classification Dose-Response Relationship Drug Chemistry Cell Biology General Medicine Rats Comet assay Oxidative Stress Treatment Outcome Enzyme Liver Cytoprotection Giant cell Silybin Female Chemical and Drug Induced Liver Injury Reactive Oxygen Species Oxidative stress Silymarin medicine.drug |
| Zdroj: | Cell Biochemistry and Biophysics. 71:1215-1223 |
| ISSN: | 1559-0283 1085-9195 |
| Popis: | Itraconazole (ITZ) belongs to the triazole group of antifungals with potent keratinophilic and lipophilic features. Hepatotoxicity is one of its most remarkable features. Silibinin (SIL) is a plant used worldwide which is used in the treatment of many liver diseases and it is especially very well known for its hepatoprotective-cytoprotective effect. The aim of our study was to research the protective effect of SIL in ITZ-induced hepatotoxicity using biochemical and pathological tests. Liver enzymes and antioxidant enzyme activities were measured spectrophotometrically by using commercial kits. ALT and AST levels in ITZ group were significantly increased compared to the group, while the activities of GSH-Px and SOD had decreased (p 0.05). When ITZ group was compared to ITZ + SIL group, AST, ALT, and levels of NO and MPO were significantly decreased, while the activities of GSH-Px and SOD were increased (p 0.05). Histopathological evaluation showed that SIL significantly decreased periportal inflammation and parenchymal hepatocyte apoptosis in ITZ and ITZ + SIL groups (p 0.05). Eventhough not statistically significant, partial improvement with the use of SIL has been detected (p 0.05) in hepatocyte degeneration and multinuclear giant cell formation. According to the evaluation performed with comet assay method, ITZ leads to DNA damage, and the use of SIL significantly decreases DNA damage (p 0.05). We have detected that the use of ITZ increases oxidative stress (MPO, NO), decreases antioxidant activity (SOD and GSH-Px), and leads to DNA damage and histopathological liver damage, whereas the use of SIL has a cytoprotective effect on the liver by increasing the antioxidant effect (SOD, GSH-Px) and by decreasing the oxidative stress (NO, MPO). ITZ causes the generation of ROS and leads to DNA damage and liver damage. SIL has a cytoprotective effect on the liver by increasing antioxidant enzyme activities, preventing the formation of ROS. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full text from SpringerLink