6,7-Dehydroroyleanone diterpene derived from Tetradenia riparia essential oil modulates IL-4/IL-12 release by macrophages that are infected with Leishmania amazonensis
| Autor: | Pollyanna Rina Ferreira da Silva, Mariana de Souza Terron-Monich, Zilda Cristiani Gazim, Maria Valdrinez Campana Lonardoni, Áquila Carolina Fernandes Herculano Ramos-Milaré, Thaís Gomes Verzignassi Silveira, Izabel Galhardo Demarchi |
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| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
medicine.medical_treatment Antiprotozoal Agents Leishmaniasis Cutaneous Pharmacology Biology Mice 03 medical and health sciences 0302 clinical medicine Cutaneous leishmaniasis In vivo Oils Volatile medicine Animals Humans Macrophage Cytotoxic T cell Interleukin 4 Leishmania Lamiaceae General Veterinary Macrophages General Medicine medicine.disease biology.organism_classification Interleukin-12 RAW 264.7 Cells 030104 developmental biology Infectious Diseases Cytokine Insect Science Abietanes Interleukin 12 Parasitology Interleukin-4 030215 immunology |
| Zdroj: | Parasitology Research. 118:369-376 |
| ISSN: | 1432-1955 0932-0113 |
| DOI: | 10.1007/s00436-018-6166-2 |
| Popis: | The treatment of cutaneous leishmaniasis in associated with several adverse effects and therapeutic failure, resulting in patients' abandonment of treatment. Research on new drugs with leishmanicidal potential from medicinal plants is essential. The anti-Leishmania activity of Tetradenia riparia essential oil (TrEO) and its derivatives, such as the diterpene 6,7-dehydroroyleanone (TrROY), and the immunomodulatory effects of TrEO have been reported. However, few studies have investigated the effects of TrROY. The present study evaluated the modulation of cytokine production by murine macrophages that were infected with Leishmania amazonensis (6 parasites/macrophage) and treated with TrROY (0.1, 1, and 100 μg/ml). Cytokine levels were measured by flow cytometry. The results were analyzed using Student's t test at a 95% confidence interval. Microscopic counting was performed to evaluate the inhibitory effects of TrROY on intracellular infection. TrROY modulated the production of cytokines that are essential for the immune defense response to Leishmania, with a decrease in interleukin-4 (IL-4) levels and an increase in IL-12 levels. A TrROY concentration of 0.1 μg/ml was chosen for the subsequent experiments. This dose was chosen because it modulated IL-4/IL-12 release by murine macrophages that were infected with Leishmania and because it presented no cytotoxic effects. TrROY (0.1 μg/ml) induced a 31% reduction of the rate of infection in murine macrophages compared with untreated cells. TrROY may be a promising leishmanicidal agent. Further in vitro and in vivo studies should be conducted to evaluate the anti-Leishmania and immunomodulatory activity of TrROY. |
| Databáze: | OpenAIRE |
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