MiR-23a targets RUNX2 and suppresses ginsenoside Rg1-induced angiogenesis in endothelial cells
| Autor: | Kun Zhang, Chao Wang, Xiao-Dong Wu, Feng Wang, Han-Qiang Liu, Li Liu, Wen-Dong Bai, Ting Guo, Zhang Mengyao |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Angiogenesis runt-related transcription factor 2 angiogenesis 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine medicine Zebrafish miR-23a vascular endothelial growth factor biology Traditional medicine business.industry Cancer biology.organism_classification medicine.disease Molecular medicine Vascular endothelial growth factor RUNX2 ginsenoside Rg1 030104 developmental biology Oncology chemistry 030220 oncology & carcinogenesis embryonic structures Cancer cell Cancer research Translational science business Research Paper |
| Zdroj: | Oncotarget |
| ISSN: | 1949-2553 |
| Popis: | // Xiao-Dong Wu 1, * , Ting Guo 4, * , Li Liu 5, * , Chao Wang 5 , Kun Zhang 1, 6 , Han-Qiang Liu 4 , Feng Wang 4 , Wen-Dong Bai 7 and Meng-Yao Zhang 1, 2, 3 1 Department of Cell Biology, National Translational Science Center for Molecular Medicine, Fourth Military Medical University, Xi’an 710032, China 2 Beijing Institute of Biotechnology, Beijing 100071, China 3 Department of Clinical Immunology, Xijing Hospital, Fourth Military Medical University, Xi’an 710032, China 4 Department of Nutrition and Food Hygiene, Fourth Military Medical University, Xi’an 710032, China 5 Department of Stomatology, PLA General Hospital, Beijing 100700, China 6 College of Life Science and Bioengineering, School of Science, Beijing Jiaotong University, Beijing 100044, China 7 Clinical Laboratory Medicine Center, Xinjiang Command General Hospital of PLA, Urumqi 830000, China * These authors have contributed equally to this work Correspondence to: Meng-Yao Zhang, email: dudybaby@126.com Wen-Dong Bai, email: bwddcgzl@163.com Feng Wang, email: wfeng@fmmu.edu.cn Keywords: angiogenesis, ginsenoside Rg1, miR-23a, runt-related transcription factor 2, vascular endothelial growth factor Received: May 01, 2017 Accepted: June 19, 2017 Published: July 22, 2017 ABSTRACT Rg1 is a predominant protopanaxatriol-type of ginsenoside found in Panax ginseng, and it has been shown to have anti-cancer effects in multiple types of cancer cells. However, Rg1 also induces the expression of proangiogenic factors, such as vascular endothelial growth factor (VEGF-A), in endothelial cells. Unfortunately, angiogenesis positively correlates with cancer development. In this study, we identified RUNX2 as a regulator of ginsenoside Rg1-induced angiogenesis for the first time. We found that RUNX2 was directly targeted and regulated by miR-23a. Additionally, miR-23a was shown to inhibit angiogenesis in both human umbilical vein endothelial cells (HUVECs) and in zebrafish. Furthermore, a decrease in RUNX2 expression resulted in translational repression of VEGF-A in HUVECs. Taken together, this study identified a MiR-23a/RUNX2/VEGF-A pathway in angiogenesis and shed light on the molecular mechanism of Rg1-induced angiogenesis. Thus, RUNX2 might be a potential therapeutic target in Rg1-mediated angiogenesis in cancer. |
| Databáze: | OpenAIRE |
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