Flow-cytometric detection of human anti-rat insulinoma antibodies in relation to anti-human islet cell and anti-insulin antibodies. Recognition of distinct antigens by antibodies in early type I diabetes
| Autor: | Hellmut Mehnert, Thomas Lander, Martin Held, Eberhard Standl, Christoph Nerl |
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| Rok vydání: | 1989 |
| Předmět: |
Male
endocrine system medicine.medical_specialty Insulin Antibodies medicine.medical_treatment Endocrinology Diabetes and Metabolism Enzyme-Linked Immunosorbent Assay Biology Immunofluorescence Cell Line Islets of Langerhans Antigen Reference Values Diabetes mellitus Internal medicine medicine Internal Medicine Animals Humans Autoantibodies Fluorescent Dyes geography geography.geographical_feature_category medicine.diagnostic_test Insulin Rat Insulinoma Autoantibody Adenoma Islet Cell Flow Cytometry Fluoresceins Islet medicine.disease Antibodies Anti-Idiotypic Rats Pancreatic Neoplasms Diabetes Mellitus Type 1 Endocrinology Immunology biology.protein Female Insulinoma Antibody Fluorescein-5-isothiocyanate Thiocyanates |
| Zdroj: | Diabetes. 38:1557-1566 |
| ISSN: | 0012-1797 |
| DOI: | 10.2337/diabetes.38.12.1557 |
| Popis: | Flow cytometry was recently introduced for the detection of antibodies in human serum to a cultured insulin-secreting rat insulinoma cell line (RINm5F) to investigate humoral immune reactivity in newly diagnosed type I (insulin-dependent) diabetic patients. Fifty-three patients were observed for 6–20 mo after clinical onset of diabetes with a reported duration of symptoms of 6 wk was 38%, whereas human anti-islet cell antibodies and anti-insulin antibodies became detectable in 72 and 61% of the patients, respectively. These findings are in line with previous reports of immunoprecipitation by human diabetic serums of a 64,000-Mr antigenic structure in freshly prepared rat islet cells. The results suggest a reactivity of distinct classes of antibodies in serums of patients with type I diabetes to disparate antigens on human islet cells and cloned rat insulinoma cells and, moreover, reactivity to insulin as the secreted product. Further characterization of the reacting RINmSF antigens and prospective studies in subjects at risk for diabetes are required to validate the application of RIN cells to the investigation of immune mechanisms involved in the pathogenesis of human type I diabetes. |
| Databáze: | OpenAIRE |
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