Searching for RET/PTC rearrangements and BRAF V599E mutation in thyroid aspirates might contribute to establish a preoperative diagnosis of papillary thyroid carcinoma
| Autor: | Maria João Bugalho, Evelina Mendonça, Luís G. Sobrinho, Rita Domingues |
|---|---|
| Rok vydání: | 2005 |
| Předmět: |
Proto-Oncogene Proteins B-raf
Pathology medicine.medical_specialty Histology endocrine system diseases Biopsy Fine-Needle DNA Mutational Analysis Mutation Missense Thyroid Gland Pathology and Forensic Medicine Thyroid carcinoma Fine needle aspiration cytology Humans Medicine Thyroid Neoplasms Gene Rearrangement Oncogene Proteins Base Sequence Reverse Transcriptase Polymerase Chain Reaction business.industry Proto-Oncogene Proteins c-ret Thyroid Receptor Protein-Tyrosine Kinases Genetic Alteration General Medicine Carcinoma Papillary Molecular analysis medicine.anatomical_structure Molecular Diagnostic Techniques Mutation (genetic algorithm) business |
| Zdroj: | Cytopathology. 16:27-31 |
| ISSN: | 1365-2303 0956-5507 |
| DOI: | 10.1111/j.1365-2303.2004.00223.x |
| Popis: | Objective: Searching for multiple molecular markers in thyroid aspirates appears to be a promising approach for establishing a preoperative diagnosis of papillary thyroid carcinoma (PTC). Methods: Based on this hypothesis, a total of 63 samples from 55 patients, were collected at random. RNA was extracted from the residue cells inside the needle used for fine needle aspiration cytology (FNAC) and thereafter molecular analysis was carried out both for RET rearrangements (type 1, 2, 3) and BRAF codon 599 mutation molecule. Results were compared with the cytological and histopathological diagnoses in 24 patients submitted to surgery. Results: 58% PTCs presented a genetic alteration either RET/PTC rearrangement, BRAF V599E mutation or both: three cases of PTCs (25%) presented a RET/PTC rearrangement; three cases of PTCs (25%) presented a BRAF V599E mutation and in one case (8%) both alterations were identified. Conclusions: The present results suggest that searching for multiple molecular markers in thyroid aspirates may enhance the accuracy of FNAC and refine preoperative diagnosis of PTC. |
| Databáze: | OpenAIRE |
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