CHD1L promotes EOC cell invasiveness and metastasis via the regulation of METAP2
| Autor: | Dan Xie, Tingting Sun, Yunyun Guo, Linglong Ouyang, Ze-Shan You, Yu Zheng, Liming Tian, Weipeng He, Guofen Yang, Zu Wei Zhang, Gui-ping Yang, Xiaohui Li, Huiling Lai |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
endocrine system diseases
Ovariectomy Cell Kaplan-Meier Estimate Carcinoma Ovarian Epithelial Biology CHD1L Metastasis Small hairpin RNA 03 medical and health sciences 0302 clinical medicine Ovarian cancer Cell Line Tumor Ovarian carcinoma Biomarkers Tumor medicine metastasis Humans Methionyl Aminopeptidases Gene silencing Neoplasm Invasiveness METAP2 Ovarian Neoplasms Gene Expression Profiling Ovary DNA Helicases General Medicine Middle Aged invasion medicine.disease female genital diseases and pregnancy complications Up-Regulation DNA-Binding Proteins Gene Expression Regulation Neoplastic medicine.anatomical_structure Tissue Array Analysis Gene Knockdown Techniques Cancer cell Cancer research Female 030211 gastroenterology & hepatology Research Paper Follow-Up Studies |
| Zdroj: | International Journal of Medical Sciences |
| ISSN: | 1449-1907 |
| DOI: | 10.7150/ijms.48615 |
| Popis: | Chromodomain helicase DNA binding protein 1-like (CHD1L) gene has been proposed to play an oncogenic role in human hepatocellular carcinoma. Previously we reported that CHD1L overexpression is significantly associated with the metastasis proceeding of epithelial ovarian cancer (EOC), and may predict a poor prognosis in EOC patients. However, the potential oncogenic mechanisms by which CHD1L acts in EOC remain unclear. To elucidate the oncogenic function of CHD1L, we carried out a series of in vitro assays, with effects of CHD1L ectogenic overexpression and silencing being determined in EOC cell lines (HO8910, A2780 and ES2). Real-time PCR and Western blotting analyses were used to identify potential downstream targets of CHD1L in the process of EOC invasion and metastasis. In ovarian carcinoma HO8910 cell lines, ectopic overexpression of CHD1L substantially induced the invasive and metastasis ability of the cancer cells in vitro. In contrast, knockdown of CHD1L using shRNA inhibited cell invasion in vitro in ovarian carcinoma A2780 and ES2 cell lines. We also demonstrated that methionyl aminopeptidase 2 (METAP2) was a downstream target of CHD1L in EOC, and we found a significant, positive correlation between the expression of CHD1L and METAP2 in EOC tissues (P |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|