Comparative Gene Expression Profiling Identifies Common Molecular Signatures of NF-kappa B Activation in Canine and Human Diffuse Large B Cell Lymphoma (DLBCL)
| Autor: | Elspeth Milne, Manikhandan Mudaliar, David M. Vail, Daniel Crowther, Gino Miele, Karen A. L. Tan, D. Ross Haggart, John R. Goodlad, Grant Sellar, Ilene D. Kurzman, David J. Argyle |
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| Jazyk: | angličtina |
| Rok vydání: | 2013 |
| Předmět: |
Pathology
medicine.medical_specialty Science Blotting Western Electrophoretic Mobility Shift Assay Biology MECHANISMS Transcriptome Dogs immune system diseases hemic and lymphatic diseases PROBE LEVEL DATA medicine KINASE Animals Humans Lymph node Oligonucleotide Array Sequence Analysis Canine Lymphoma Multidisciplinary Microarray analysis techniques BIOINFORMATICS NF-kappa B CHEMOTHERAPY medicine.disease Immunohistochemistry CANCER Lymphoma DISTINCT SUBGROUPS APOPTOSIS Gene expression profiling medicine.anatomical_structure Tissue Array Analysis Cancer research Medicine Lymphoma Large B-Cell Diffuse DIHYDROFOLATE-REDUCTASE DNA microarray Diffuse large B-cell lymphoma SUMMARIES Research Article |
| Zdroj: | Mudaliar, M A V, Haggart, R D, Miele, G, Sellar, G, Tan, K, Goodlad, J, Milne, E, Vail, D M, Kurzman, I, Crowther, D & Argyle, D J 2013, ' Comparative Gene Expression Profiling Identifies Common Molecular Signatures of NF-kappa B Activation in Canine and Human Diffuse Large B Cell Lymphoma (DLBCL) ', PLoS ONE, vol. 8, no. 9, e72591 . https://doi.org/10.1371/journal.pone.0072591 PLoS ONE, Vol 8, Iss 9, p e72591 (2013) PLoS ONE |
| DOI: | 10.1371/journal.pone.0072591 |
| Popis: | We present the first comparison of global transcriptional changes in canine and human diffuse large B-cell lymphoma (DLBCL), with particular reference to the nuclear factor-kappa B (NF-kappa'B) pathway. Microarray data generated from canine DLBCL and normal lymph nodes were used for differential expression, co-expression and pathway analyses, and compared with analysis of microarray data from human healthy and DLBCL lymph nodes. The comparisons at gene level were performed by mapping the probesets in canine microarrays to orthologous genes in humans and vice versa. A considerable number of differentially expressed genes between canine lymphoma and healthy lymph node samples were also found differentially expressed between human DLBCL and healthy lymph node samples. Principal component analysis using a literature-derived NF-kappa B target gene set mapped to orthologous canine array probesets and human array probesets clearly separated the healthy and cancer samples in both datasets. The analysis demonstrated that for both human and canine DLBCL there is activation of the NF-kappa B/p65 canonical pathway, indicating that canine lymphoma could be used as a model to study NF-kappa B-targeted therapeutics for human lymphoma. To validate this, tissue arrays were generated for canine and human NHL and immunohistochemistry was employed to assess NF-kappa B activation status. In addition, human and canine B-cell lymphoma lines were assessed for NF-kB activity and the effects of NF-kappa B inhibition. |
| Databáze: | OpenAIRE |
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