Generation of a genetically modified chimeric plasmodium falciparum parasite expressing plasmodium vivax circumsporozoite protein for malaria vaccine development
| Autor: | Takashi Imai, Rianne van der Laak, Fiona J. A. Geurten, Surendra Kumar Kolli, Miguel Prudêncio, Yukiko Miyazaki, Chris J. Janse, Brandon K. Wilder, Shahid M. Khan, Catherin Marin-Mogollon, Jai Ramesar, Shinya Miyazaki, Hans Kroeze, Koen J. Dechering, Arturo Reyes-Sandoval, Angelika Sturm, Roos van Schuijlenburg, Ahmed M. Salman, Blandine Franke-Fayard, António M. Mendes, Severine Chevalley-Maurel |
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| Přispěvatelé: | Repositório da Universidade de Lisboa |
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Microbiology (medical) Immunology Plasmodium vivax Plasmodium falciparum lcsh:QR1-502 Protozoan Proteins malaria Antibodies Protozoan Biology Microbiology lcsh:Microbiology Mice 03 medical and health sciences 0302 clinical medicine Cellular and Infection Microbiology Chimeric parasites Malaria Vaccines parasitic diseases medicine Animals 030212 general & internal medicine Malaria Falciparum Original Research Vaccines Malaria vaccine circumsporozoite protein (CSP) vaccines Circumsporozoite protein (CSP) medicine.disease biology.organism_classification Virology Malaria Genetically modified organism Circumsporozoite protein 030104 developmental biology Infectious Diseases Immunization biology.protein chimeric parasites Antibody |
| Zdroj: | Frontiers in Cellular and Infection Microbiology, 10. FRONTIERS MEDIA SA Frontiers in Cellular and Infection Microbiology Repositório Científico de Acesso Aberto de Portugal Repositório Científico de Acesso Aberto de Portugal (RCAAP) instacron:RCAAP Frontiers in Cellular and Infection Microbiology, Vol 10 (2020) |
| ISSN: | 9550-2017 |
| DOI: | 10.3389/fcimb.2020.591046/full |
| Popis: | Copyright © 2020 Miyazaki, Marin-Mogollon, Imai, Mendes, van der Laak, Sturm, Geurten, Miyazaki, Chevalley-Maurel, Ramesar, Kolli, Kroeze, van Schuijlenburg, Salman, Wilder, Reyes-Sandoval, Dechering, Prudencio, Janse, Khan and ̂ Franke-Fayard. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. Chimeric rodent malaria parasites with the endogenous circumsporozoite protein (csp) gene replaced with csp from the human parasites Plasmodium falciparum (Pf) and P. vivax (Pv) are used in preclinical evaluation of CSP vaccines. Chimeric rodent parasites expressing PfCSP have also been assessed as whole sporozoite (WSP) vaccines. Comparable chimeric P. falciparum parasites expressing CSP of P. vivax could be used both for clinical evaluation of vaccines targeting PvCSP in controlled human P. falciparum infections and in WSP vaccines targeting P. vivax and P. falciparum. We generated chimeric P. falciparum parasites expressing both PfCSP and PvCSP. These Pf-PvCSP parasites produced sporozoite comparable to wild type P. falciparum parasites and expressed PfCSP and PvCSP on the sporozoite surface. Pf-PvCSP sporozoites infected human hepatocytes and induced antibodies to the repeats of both PfCSP and PvCSP after immunization of mice. These results support the use of Pf-PvCSP sporozoites in studies optimizing vaccines targeting PvCSP. CM-M was, in part, supported by Colciencias Ph.D. fellowship (Call 568 from 2012 Resolution 01218 Bogotá, Colombia). TI was, in part, supported by Uehara Memorial Foundation grant. Work performed at IMM was supported by Fundação para a Ciência e Tecnologia (FCT-Portugal)’s grants PTDC/BBB-BMD/2695/2014 and PTDC-SAU-INF-29550-2017. AR-S is supported by the MRC-DPFS grant MR/N019008/1. |
| Databáze: | OpenAIRE |
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