Green tea polyphenol 'epigallocatechin-3-gallate', differentially induces apoptosis in CLL B-and T-Cells but not in healthy B-and T-Cells in a dose dependant manner
| Autor: | Reza Ghassemifar, David Joske, Scott Cornwall, Gavin Cull |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Cancer Research Leukemia T-Cell Stromal cell T-Lymphocytes Cell Apoptosis Biology Catechin Flow cytometry 03 medical and health sciences 0302 clinical medicine immune system diseases hemic and lymphatic diseases White blood cell Tumor Cells Cultured medicine Humans Cells Cultured B-Lymphocytes Dose-Response Relationship Drug medicine.diagnostic_test Feeder Cells Hematology Leukemia Lymphocytic Chronic B-Cell Coculture Techniques In vitro 030104 developmental biology medicine.anatomical_structure Oncology Cell culture 030220 oncology & carcinogenesis Immunology Monoclonal Cancer research |
| Zdroj: | Leukemia Research. 51:56-61 |
| ISSN: | 0145-2126 |
| DOI: | 10.1016/j.leukres.2016.10.011 |
| Popis: | B-cell chronic lymphocytic leukaemia (CLL) is characterized by an accumulation of CD5-positive monoclonal B-cells due in large part to a failure of apoptosis. The ability to study CLL B-cells in vitro has always been a challenge and hampered by the low viability of the CLL B-cells in cell culture systems. In this study, we present a multicellular cell culture system to maintain CLL B-cells viable in culture for 60h in the presence of a stromal cell feeder layer in combination with a whole white blood cell preparation. Using this optimized system, we tested and showed that the addition of epigallocatechin-3-gallate (EGCG) at concentrations ranging from 25 to 100μg/ml induced apoptosis in CLL B-cells whilst not affecting healthy control B-cells. Moreover, the results showed that in contrast to healthy controls, T-cells from CLL patients underwent apoptosis in the presence of EGCG. This study demonstrated that the combination of a cell feeder layer with a whole white blood cell preparation maintained B-cell viability in vitro over an extended period of time. In addition, the study showed that EGCG differentially induces apoptosis in CLL B-and T-Cells but not in healthy B-and T-Cells in a dose dependent manner. |
| Databáze: | OpenAIRE |
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