| Popis: |
The synthesis of virus-specific RNA has been studied in ascites tumor cells infected with Maus-Elberfeld virus. Analysis by sucrose density centrifugation revealed a large 35 s component of intact viral RNA plus a smaller amount of slower sedimenting virus-specific RNA, part of which was resistant to hydrolysis by RNase. Methods were developed for fractionating the infected ascites cells into cytoplasmic and nuclear components. At all times following infection, more than 90% of the virus-specific RNA was found in the cytoplasmic fraction from the cells. Of this material, approximately 80% of the total virus-specific RNA and all of the RNase-resistant RNA appeared to be associated with cytoplasmic membranes, much of which adhered tenaciously to the nuclei. |
