Importance of growth form on production of hybrid antibiotic byStreptomyces lividans TK21 by fed-batch and continuous fermentation
| Autor: | Josep A. Pérez-Pons, Montserrat Sarrà, Francesc Gòdia, Carlos Casas Alvero |
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| Rok vydání: | 1998 |
| Předmět: |
Continuous operation
Biomass Bioengineering Applied Microbiology and Biotechnology Biochemistry Microbiology Bioreactors Transformation Genetic Bioreactor Food science Molecular Biology biology Streptomycetaceae General Medicine biology.organism_classification Streptomyces Anti-Bacterial Agents Culture Media Kinetics Chemically defined medium Fermentation Actinomycetales Bacteria Plasmids Biotechnology |
| Zdroj: | Applied Biochemistry and Biotechnology. 75:235-248 |
| ISSN: | 1559-0291 0273-2289 |
| DOI: | 10.1007/bf02787777 |
| Popis: | A fermentation strategy, based on the controlled feeding of growth-limiting nutrients in order to maintain metabolic activity for extended periods, has been examined in the case of the production of a hybrid antibiotic by a transformed strain of Streptomyces lividans TK21. The fed-batch operation did not improve the results obtained with batch operation. Continuous cultures on defined medium showed stable levels of biomass concentration, but antibiotic production ceased when continuous operation was started. The results obtained indicate the critical influence that morphology of the cell aggregates has on metabolic activity. The antibiotic is produced only in culture conditions providing growth in compact mycelial pellets. |
| Databáze: | OpenAIRE |
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