Targeting microglia with lentivirus and AAV: Recent advances and remaining challenges
| Autor: | Rouven Schulz, Sandra Siegert, Gloria Colombo, Margaret E. Maes |
|---|---|
| Jazyk: | angličtina |
| Předmět: |
0301 basic medicine
Future studies viruses Genetic Vectors Adeno-associated virus (AAV) Functional impact Disease Article Viral vector 03 medical and health sciences Transduction (genetics) 0302 clinical medicine Transduction Genetic Research community medicine Animals Humans biology Microglia General Neuroscience Lentivirus Brain Dependovirus biology.organism_classification 3. Good health 030104 developmental biology medicine.anatomical_structure nervous system Gene Targeting HIV-1 In-vivo transduction Neuroscience 030217 neurology & neurosurgery |
| Zdroj: | Neuroscience Letters |
| ISSN: | 0304-3940 |
| DOI: | 10.1016/j.neulet.2019.134310 |
| Popis: | Highlights • Background of lentiviral and adeno-associated virus vector design. • Reviewing of literature that reported in vivo microglial transduction. • Challenges to overcome low microglial transduction efficiency and specificity. • Guidelines for reporting viral transduction in microglia. Microglia have emerged as a critical component of neurodegenerative diseases. Genetic manipulation of microglia can elucidate their functional impact in disease. In neuroscience, recombinant viruses such as lentiviruses and adeno-associated viruses (AAVs) have been successfully used to target various cell types in the brain, although effective transduction of microglia is rare. In this review, we provide a short background of lentiviruses and AAVs, and strategies for designing recombinant viral vectors. Then, we will summarize recent literature on successful microglial transductions in vitro and in vivo, and discuss the current challenges. Finally, we provide guidelines for reporting the efficiency and specificity of viral targeting in microglia, which will enable the microglial research community to assess and improve methodologies for future studies. |
| Databáze: | OpenAIRE |
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