Planarian MBD2/3 is required for adult stem cell pluripotency independently of DNA methylation
| Autor: | Sunir Malla, A. Aziz Aboobaker, Belen Tejada Romero, Priscilla J.K.P. Lo, Jordi Solana, Yuliana Mihaylova, Jack S. Benner, Chen Chen, Alexey Ruzov, Jeremy M. Foster, Farah Jaber-Hijazi |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2013 |
| Předmět: |
Pluripotency
Pluripotent Stem Cells Rex1 Cellular differentiation 03 medical and health sciences 0302 clinical medicine Regeneration Animals Induced pluripotent stem cell Evolution of Developmental Control Mechanisms Molecular Biology Tissue homeostasis 030304 developmental biology Genetics 0303 health sciences biology Cell Differentiation Planarians Cell Biology DNA Methylation biology.organism_classification Methyl binding domain Planarian Differentiation DNA methylation 5-Methylcytosine Stem cell 030217 neurology & neurosurgery Adult stem cell Developmental Biology |
| Zdroj: | Developmental Biology |
| ISSN: | 1095-564X 0012-1606 |
| Popis: | Planarian adult stem cells (pASCs) or neoblasts represent an ideal system to study the evolution of stem cells and pluripotency as they underpin an unrivaled capacity for regeneration. We wish to understand the control of differentiation and pluripotency in pASCs and to understand how conserved, convergent or divergent these mechanisms are across the Bilateria. Here we show the planarian methyl-CpG Binding Domain 2/3 (mbd2/3) gene is required for pASC differentiation during regeneration and tissue homeostasis. The genome does not have detectable levels of 5-methylcytosine (5mC) and we find no role for a potential DNA methylase. We conclude that MBD proteins may have had an ancient role in broadly controlling animal stem cell pluripotency, but that DNA methylation is not involved in planarian stem cell differentiation. Highlights • A single ancestral MBD2/3 protein is present in the planarian Schmidtea mediterranea. • The genome of S. mediterranea does not have pervasive cytosine methylation. • MBD2/3 is required for pluripotent stem cell differentiation down multiple but not all cell lineages. • MBD2/3 may have had an ancestral role in regulating stem cell pluripotency. |
| Databáze: | OpenAIRE |
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