From birth to adulthood: An analysis of the Brazilian lancehead (Bothrops moojeni) venom at different life stages

Autor: Caroline Serino-Silva, Alexandre Keiji Tashima, Erika S. Nishiduka, Kathleen Fernandes Grego, Caroline Fabri Bittencourt Rodrigues, Daniela Miki Hatakeyama, Anita Mitico Tanaka-Azevedo, Nathália da Costa Galizio, Daniel Rodrigues Stuginski, Sávio Stefanini Sant'Anna, Karen de Morais-Zani, Lídia Jorge Tasima
Jazyk: angličtina
Rok vydání: 2021
Předmět:
0301 basic medicine
Male
Hydrolases
Ontogeny
Venom
Reptilian Proteins
Phospholipase
Toxicology
Pathology and Laboratory Medicine
Median lethal dose
Biochemistry
Vascular Medicine
Geographical locations
Mass Spectrometry
Bothrops moojeni
Medicine and Health Sciences
Toxins
Bothrops
Chromatography
High Pressure Liquid
Multidisciplinary
biology
Esterases
Eukaryota
Gene Expression Regulation
Developmental
Snakes
Proteases
Squamates
Enzymes
Phospholipases
Vertebrates
Medicine
Female
Brazil
Research Article
Electrophoresis
Science
Toxic Agents
Zoology
Hemorrhage
Age and sex
L-Amino Acid Oxidase
complex mixtures
03 medical and health sciences
Signs and Symptoms
Crotalid Venoms
Animals
030102 biochemistry & molecular biology
Venoms
Organisms
Biology and Life Sciences
Reptiles
Proteins
Neonates
South America
biology.organism_classification
Life stage
Phospholipases A2
030104 developmental biology
Amniotes
Enzymology
Metalloproteases
Clinical Medicine
Serine Proteases
People and places
Developmental Biology
Zdroj: PLoS ONE, Vol 16, Iss 6, p e0253050 (2021)
PLoS ONE, Vol 16, Iss 6 (2021)
PLoS ONE
ISSN: 1932-6203
Popis: The Brazilian lancehead (Bothrops moojeni) has a wide distribution in Brazil and represents a serious public health hazard. Previous works reported that the symptoms of snakebites caused by B. moojeni juveniles’ bites were mainly related to coagulation, while those caused by adults’ bites had a more prominent local damage. In this work, we analyzed the venoms of B. moojeni at different life stages to better understand the ontogeny shift in this species. Snakes were grouped by age and sex, and venom pools were formed accordingly. Compositional analyses by one-dimensional electrophoresis (1-DE), chromatography, and mass spectrometry revealed that ontogenetic changes might be mostly related to phospholipase A2 (PLA2) and metalloproteases. Regarding the venoms functional aspect, proteolytic, L-amino acid oxidase, PLA2, and coagulant in vitro activities were assayed, but only the first and the last ones showed age-related changes, with the venom of snakes up to 1 year-old displaying lower proteolytic and higher coagulant activities, while those from 2 years-old onward presented the opposite relation. The venoms of 3 years-old snakes were exceptions to the compositional and functional pattern of adults as both venoms presented profiles similar to neonates. Sex-related differences were observed in specific groups and were not age-related. In vivo experiments (median lethal dose and hemorrhagic activity) were statistically similar between neonates and adults, however we verified that the adult venom killed mice faster comparing to the neonates. All venoms were mostly recognized by the antibothropic serum and displayed similar profiles to 1-DE in western blotting. In conclusion, the Brazilian lancehead venom showed ontogenetic shift in its composition and activities. Furthermore, this change occurred in snakes from 1 to 2 years-old, and interestingly the venom pools from 3 years-old snakes had particular characteristics, which highlights the importance of comprehensive studies to better understand venom variability.
Databáze: OpenAIRE
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