Methylmercury Determination in Fish by Direct Mercury Analyzer
| Autor: | Jeong-Yun Hwang, Sheen-Hee Kim, Min-Ja Cho, Gil-Jin Kang, Hye-Eun Lee, Taehun Kim, Ji Hui Kim, Yongmin Lee, Jangduck Choi |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
Pharmacology
Detection limit Spectrum analyzer Chromatography Aqueous medium 010405 organic chemistry Chemistry 010401 analytical chemistry Fishes chemistry.chemical_element Mercury Methylmercury Compounds 01 natural sciences 0104 chemical sciences Analytical Chemistry Mercury (element) chemistry.chemical_compound Certified reference materials Total hg Animals Environmental Chemistry Agronomy and Crop Science Methylmercury Food Science |
| Zdroj: | Journal of AOAC INTERNATIONAL. 103:244-249 |
| ISSN: | 1944-7922 1060-3271 |
| Popis: | Background: There are two methods for quantifying methylmercury (MeHg) in fish using GC–electron capture detection (ECD): AOAC INTERNATIONAL Official Method 988.11 and the Korean Food Code (KFC) method. Both of these methods consume a large amount of chemicals and require long pretreatment times because of several complicated MeHg extraction steps. Objective: In this study, a new method for the simple and rapid determination of MeHg in fish has been developed. The method is based on the investigation of oxygen combustion–gold amalgamation using a direct mercury analyzer (DMA) after the complete removal of MeHg by organic extraction and back-extraction to an aqueous medium. Methods: The DMA is suitable for the analysis of both solid and liquid materials and has a good detection limit. The developed method was validated by comparing the MeHg recoveries (%) of both certified reference materials and the market-purchased fish samples with the MeHg concentration obtained using the KFC method. Results: The following parameters pertaining to the developed method were established: detection limit, 1.02 μg/kg; LOQ, 3.09 μg/kg; linearity (r), 0.9998; range, 0.1–300 μg/kg; and recovery, 95–97%. Conclusions: Our method is a promising alternative by virtue of its much simpler and faster sample pretreatment procedure, with a MeHg recovery as high as that of the KFC method. Highlights: The developed method enables the simultaneous analysis of total Hg and MeHg with only DMA equipment. |
| Databáze: | OpenAIRE |
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