Real-Time Multiplex PCR Assay for Detection of Brucella spp., B. abortus , and B. melitensis
| Autor: | Susan L. Bystrom, Margot H. Graves, Nhi Y. Khuong, Kimmi N. Schrader, William S. Probert |
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| Rok vydání: | 2004 |
| Předmět: |
Microbiology (medical)
Pcr assay Brucella abortus Brucellaceae Brucella Polymerase Chain Reaction law.invention Microbiology Single test Computer Systems law RNA Ribosomal 16S Multiplex polymerase chain reaction Brucella melitensis Multiplex Polymerase chain reaction DNA Primers Base Sequence biology Bacteriology bacterial infections and mycoses biology.organism_classification Virology RNA Bacterial |
| Zdroj: | Journal of Clinical Microbiology. 42:1290-1293 |
| ISSN: | 1098-660X 0095-1137 |
| DOI: | 10.1128/jcm.42.3.1290-1293.2004 |
| Popis: | The identification of Brucella can be a time-consuming and labor-intensive process that places personnel at risk for laboratory-acquired infection. Here, we describe a real-time PCR assay for confirmation of presumptive Brucella isolates. The assay was designed in a multiplex format that will allow the rapid identification of Brucella spp., B. abortus , and B. melitensis in a single test. |
| Databáze: | OpenAIRE |
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