Localization of the GABAergic and non-GABAergic neurons projecting to the sublaterodorsal nucleus and potentially gating paradoxical sleep onset

Autor:	Damien Gervasoni, Romuald Boissard, Patrice Fort, Pierre-Hervé Luppi, Bruno Barbagli
Přispěvatelé:	Trelleborg, Centre de recherche en neurosciences de Lyon (CRNL), Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Université Jean Monnet [Saint-Étienne] (UJM)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Physio-pathologie des réseaux neuronaux du cycle veille-sommeil, Centre National de la Recherche Scientifique (CNRS)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon, Team Physiopathologie des Réseaux Neuronaux Responsables du Cycle Veille-Sommeil, Université de Lyon-Université de Lyon-Université Jean Monnet [Saint-Étienne] (UJM)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Université Claude Bernard Lyon 1 (UCBL)
Rok vydání:	2003
Předmět:	Male [SDV]Life Sciences [q-bio] [SDV.NEU.NB]Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC]/Neurobiology MESH: Neurons MESH: Rats Sprague-Dawley MESH: gamma-Aminobutyric Acid Rats Sprague-Dawley 0302 clinical medicine Neural Pathways MESH: Animals ComputingMilieux_MISCELLANEOUS gamma-Aminobutyric Acid Neurons Medulla Oblongata 0303 health sciences Glutamate Decarboxylase Chemistry General Neuroscience Central nucleus of the amygdala MESH: Comparative Study Electroencephalography Iontophoresis Immunohistochemistry MESH: Research medicine.anatomical_structure GABAergic [SDV.NEU]Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC] MESH: Iontophoresis medicine.drug MESH: Glutamate Decarboxylase Cholera Toxin MESH: Rats Sleep REM MESH: Phytohemagglutinins MESH: Electromyography 03 medical and health sciences Glutamatergic MESH: Medulla Oblongata MESH: Electroencephalography medicine Animals Phytohemagglutinins MESH: Cholera Toxin 030304 developmental biology Electromyography MESH: Neural Pathways MESH: Immunohistochemistry Bicuculline MESH: Sleep REM MESH: Male Rats Stria terminalis nervous system Reticular connective tissue Gabazine Neuroscience Nucleus 030217 neurology & neurosurgery
Zdroj:	Supplement ... to the European journal of neuroscience. Supplement .. to the European journal of neuroscience., 2003, 18 (6), pp.1627-39 European Journal of Neuroscience European Journal of Neuroscience, Wiley, 2003, 18 (6), pp.1627-39 European Journal of Neuroscience, Wiley, 2003, 18 (6), pp.1627-1639. ⟨10.1046/j.1460-9568.2003.02861.x⟩
ISSN:	1460-9568 0953-816X 1359-5962
DOI:	10.1046/j.1460-9568.2003.02861.x
Popis:	International audience; We recently determined in rats that iontophoretic application of bicuculline or gabazine [two GABAa antagonists] and kainic acid (a glutamate agonist) in the sublaterodorsal nucleus (SLD) induces with a very short latency a paradoxical sleep-like state. From these results, we proposed that GABAergic and glutamatergic inputs to the SLD paradoxical sleep (PS)-executive neurons gate the onset of PS [R. Boissard et al. (2002) Eur. J. Neurosci., 16, 1959-1973]. We therefore decided to determine the origin of the GABAergic and non-GABAergic inputs to the SLD combining ejection of a retrograde tracer [cholera-toxin B subunit (CTb)] with glutamate decarboxylase (GAD) immunohistochemistry. The presence of GAD-immunoreactive neurons in the SLD was confirmed. Then, following CTb ejections centred on the SLD, combined with GAD and CTb immunohistochemistry, double-labelled cells were observed in the mesencephalic and pontine reticular nuclei and to a lesser extent the parvicellular reticular nucleus. A large number of GAD-negative retrogradely labelled cells was also seen in these structures as well as in the primary motor area of the frontal cortex, the central nucleus of the amygdala, the ventral and lateral bed nucleus of the stria terminalis, the lateral hypothalamic area, the lateral and ventrolateral periaqueductal grey and the lateral paragigantocellular reticular nucleus. From these results, we propose that the activation of PS-executive neurons from the SLD is due to the removal of a tonic inhibition from GABAergic neurons localized in the SLD, and the mesencephalic and pontine reticular nuclei. Strong non-GABAergic inputs to the SLD could be excitatory and responsible for the tonic glutamatergic input on the PS-on neurons we have previously described. They could also terminate on SLD GABAergic interneurons and be indirectly responsible for the inhibition of the PS-on neurons during waking and slow-wave sleep.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::160653d3207b019ed52ab450c2f6968b https://doi.org/10.1046/j.1460-9568.2003.02861.x Zobrazit plný text záznamu Plný text ve formátu PDF Plný text ve formátu HTML
Nepřihlášeným uživatelům se plný text nezobrazuje	K zobrazení výsledku je třeba se přihlásit.