Rapid isolation and detection of erythropoietin in blood plasma by magnetic core gold nanoparticles and portable Raman spectroscopy
| Autor: | Arumugam Sivanesan, Emad L. Izake, Rohan Steel, Roland Agoston, Martin N. Sillence, William B. Lott |
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| Rok vydání: | 2016 |
| Předmět: |
Materials science
Biomedical Engineering Pharmaceutical Science Medicine (miscellaneous) Nanoparticle Bioengineering Nanotechnology 02 engineering and technology Spectrum Analysis Raman 01 natural sciences symbols.namesake Blood plasma Protein purification Animals Humans General Materials Science Horses Magnetite Nanoparticles Erythropoietin Chromatography 010401 analytical chemistry Substrate (chemistry) Surface-enhanced Raman spectroscopy 021001 nanoscience & nanotechnology 0104 chemical sciences Substance Abuse Detection Magnetic core Colloidal gold Magnets symbols Molecular Medicine Gold 0210 nano-technology Raman spectroscopy Antibodies Immobilized |
| Zdroj: | Nanomedicine: Nanotechnology, Biology and Medicine. 12:633-641 |
| ISSN: | 1549-9634 |
| Popis: | Isolating, purifying, and identifying proteins in complex biological matrices are often difficult, time consuming, and unreliable. Herein we describe a rapid screening technique for proteins in biological matrices that combines selective protein isolation with direct surface enhanced Raman spectroscopy (SERS) detection. Magnetic core gold nanoparticles were synthesized, characterized, and subsequently functionalized with recombinant human erythropoietin (rHuEPO)-specific antibody. The functionalized nanoparticles were used to capture rHuEPO from horse blood plasma within 15 min. The selective binding between the protein and the functionalized nanoparticles was monitored by SERS. The purified protein was then released from the nanoparticles' surface and directly spectroscopically identified on a commercial nanopillar SERS substrate. ELISA independently confirmed the SERS identification and quantified the released rHuEPO. Finally, the direct SERS detection of the extracted protein was successfully demonstrated for in-field screening by a handheld Raman spectrometer within 1 min sample measurement time.The rapid detection of recombinant human erythropoietin (rHuEPO) is important in competitive sports to screen for doping offences. In this article, the authors reported their technique of direct surface enhanced Raman spectroscopy (SERS) detection using magnetic core gold nanoparticles functionalized with recombinant human erythropoietin-specific antibody. The findings should open a new way for future detection of other proteins. |
| Databáze: | OpenAIRE |
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